Structural changes and neurotrophic factors upregulation in submandibular gland in a rat model of depression: proposed correlation with stress indicators during and after the relief of depression.

Autor: Eldomiaty MA; Department of Anatomy and Histology, Faculty of Medicine, Al-Rayan Medical Colleges, Medina, Saudi Arabia. Ma.eldomiaty@amc.edu.sa.; Department of Anatomy, Faculty of Medicine, Tanta University, Tanta, Egypt. Ma.eldomiaty@amc.edu.sa., A Hassan Z; Department of Anatomy, Faculty of Medicine, Taibah University, Medina, Saudi Arabia.; Faculty of Medicine, Histology and Cell Biology, Zagazig University, Zagazig, Egypt., Halawa AM; Department of Anatomy, Faculty of Medicine, Tanta University, Tanta, Egypt., Elnajar AM; Faculty of Dentistry, Misr University of Science and Technology, Giza, Egypt., Almohamadi N; Department of Pathology, Faculty of Medicine, Taibah University, Medina, Saudi Arabia.
Jazyk: angličtina
Zdroj: Anatomical science international [Anat Sci Int] 2023 Mar; Vol. 98 (2), pp. 185-195. Date of Electronic Publication: 2022 Oct 01.
DOI: 10.1007/s12565-022-00686-3
Abstrakt: This study evaluated the structural changes, the immunohistochemical and gene expression of neurotrophic factors in submandibular gland in a rat model of depression, and their correlation with depression parameters during and after relief of depression by voluntary running. Forty-eight male Wistar rats were divided into control, control-exercise, depression, and depression-exercise groups. Depression was induced using forced swimming protocol, while the relief of depression was induced using the rat voluntary running wheels. The depressive state of rats was evaluated by measuring the immobility duration and the serum corticosterone level. The immune expression was evaluated by measuring the optical densities (ODs) using ImageJ software, and the gene expression levels were investigated. In the depression group, the convoluted ducts appeared dilated with numerous secretory granules. The number of PCNA-stained cells was significantly decreased in the depression group as compared to control group and then significantly increased in the depression-exercise group when compared to the depression group with a negative correlation to stress indicator. The ODs of immuno-expression for the brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) increased significantly in the depression group as compared to control group. Both BDNF and VEGF immuno-expression displayed positive correlation with the stress indicators. Both BDNF and VEGF gene expression results confirmed their immunohistochemical results. The findings of this study explored the role of submandibular gland in secreting neurotrophic factors and raise a flag for the possibility of using salivary secretions as dependable and easy parameter for estimation of chronic stressed patients.Mini AbstractThe submandibular gland neurotrophic factors immuno-expression can be used in estimating chronic depressive disorders as they are correlated with stress indicators during and after the relief of depression.
(© 2022. The Author(s), under exclusive licence to Japanese Association of Anatomists.)
Databáze: MEDLINE
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