Organoids as tools for fundamental discovery and translation-a Keystone Symposia report.

Autor: Cable J; PhD Science Writer, New York City, New York, USA., Lutolf MP; Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Lausanne, Switzerland.; Institute of Chemical Sciences and Engineering, School of Basic Science (SB), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Roche Institute for Translational Bioengineering (ITB), Pharma Research and Early Development (pRED), F. Hoffmann-La Roche Ltd, Basel, Switzerland., Fu J; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.; Department of Cell & Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA., Park SE; Department of Bioengineering and NSF Science and Technology Center for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Apostolou A; Emulate Inc, Boston, Massachusetts, USA.; Department of Medicine, National and Kapodistrian University of Athens, Athens, Greece., Chen S; Department of Surgery, Weill Cornell Medical College, New York City, New York, USA., Song CJ; Keck Medicine of University of Southern California, Los Angeles, California, USA., Spence JR; Department of Cell & Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Liberali P; Friedrich Miescher Institute for Biomedical Research (FMI) and University of Basel, Basel, Switzerland., Lancaster M; MRC Laboratory of Molecular Biology, Cambridge, UK., Meier AB; First Department of Medicine, Cardiology, Klinikum rechts der Isar, Technical University of Munich, School of Medicine and Health, Munich, Germany., Pek NMQ; Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati, Ohio, USA.; Division of Pulmonary Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.; College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA., Wells JM; Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati, Ohio, USA.; Division of Developmental Biology and Division of Endocrinology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA., Capeling MM; Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, Michigan, USA., Uzquiano A; Department of Stem Cell and Regenerative Biology, Harvard University.; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Musah S; Developmental and Stem Cell Biology Program and Division of Nephrology, Department of Medicine and Department of Cell Biology, Duke University School of Medicine, Durham, North Carolina, USA.; Center for Biomolecular and Tissue Engineering, Durham, North Carolina, USA.; Department of Biomedical Engineering, Pratt School of Engineering, Durham, North Carolina, USA.; Duke Regeneration Center, Duke University, Durham, North Carolina, USA., Huch M; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany., Gouti M; Stem Cell Modelling of Development & Disease Group, Max Delbrück Center for Molecular Medicine, Berlin, Germany., Hombrink P; University Medical Center Utrecht and HUB Organoids, Utrecht, Netherlands., Quadrato G; Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine and Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research, University of Southern California, Los Angeles, California, USA., Urenda JP; Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine and Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research, University of Southern California, Los Angeles, California, USA.
Jazyk: angličtina
Zdroj: Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2022 Dec; Vol. 1518 (1), pp. 196-208. Date of Electronic Publication: 2022 Sep 30.
DOI: 10.1111/nyas.14874
Abstrakt: Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to recapitulate human physiology and pathology. Significant progress has been made in driving stem cells to differentiate into different organoid types, though several challenges remain. For example, many organoid models suffer from high heterogeneity, and it can be difficult to fully incorporate the complexity of in vivo tissue and organ development to faithfully reproduce human biology. Successfully addressing such limitations would increase the viability of organoids as models for drug development and preclinical testing. On April 3-6, 2022, experts in organoid development and biology convened at the Keystone Symposium "Organoids as Tools for Fundamental Discovery and Translation" to discuss recent advances and insights from this relatively new model system into human development and disease.
(© 2022 New York Academy of Sciences.)
Databáze: MEDLINE
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