Biochemical properties of H + -Ca 2+ -exchanger in the myometrium mitochondria.
| Autor: | Danylovych YV; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine., Danylovych HV; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine., Kolomiets OV; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine., Sviatnenko MD; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine., Kosterin SO; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine. |
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| Jazyk: | angličtina |
| Zdroj: | Current research in physiology [Curr Res Physiol] 2022 Sep 21; Vol. 5, pp. 369-380. Date of Electronic Publication: 2022 Sep 21 (Print Publication: 2022). |
| DOI: | 10.1016/j.crphys.2022.09.005 |
| Abstrakt: | Some biochemical properties of the H + -Ca 2+ -exchanger in uterine smooth muscle mitochondria have been described. The experiments were performed on a suspension of isolated mitochondria from the myometrium of rats. Methods of confocal microscopy, spectrofluorimetry and photon correlation spectroscopy were used. Fluo-4 probe was used to record changes in ionized Ca 2+ in the matrix and cytosol; pH changes in the matrix were evaluated with BCECF. It was experimentally proved that in the myometrium instead of Na + -Ca 2+ -exchanger the H + -Ca 2+ -exchanger functions. It was activated at a physiological pH value, was carried out in stoichiometry 1: 1 and was electrogenic. The transport system was modulated by magnesium ions and the diuretic amiloride, but was not sensitive to changes in the concentration of extra-mitochondrial potassium ions. H + -Ca 2+ -exchanger was suppressed by antibodies against the LETM1 protein. Calmodulin may act as a regulator of H + -Ca 2+ -exchanger by inhibiting it. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Danylovych Hanna reports financial support was provided by 10.13039/501100004742Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. (© 2022 The Authors.) |
| Databáze: | MEDLINE |
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