Anti-tumor effect of berberine on chronic lymphocytic leukemia cells.

Autor: Abdollahi M; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran.; Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran., Mohammadlou M; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran.; Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran., Hemati M; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran., Baharlou R; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran., Manouchehri Doulabi E; Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden., Ghahremanfard F; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran. f_ghahremanfard@yahoo.com., Sarabi MA; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran., Kokhaei P; Department of Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran. parviz.kokhaei@ki.se.; Department of Oncology-Pathology, BioClinicum, Karolinska University Hospital Solna and Karolinska Institutet, Stockholm, Sweden. parviz.kokhaei@ki.se.
Jazyk: angličtina
Zdroj: Medical oncology (Northwood, London, England) [Med Oncol] 2022 Sep 29; Vol. 39 (12), pp. 217. Date of Electronic Publication: 2022 Sep 29.
DOI: 10.1007/s12032-022-01818-5
Abstrakt: Chronic lymphocytic leukemia (CLL) is a blood malignancy that is characterized by remarkable expression of CD69 and Ki67 in CLL cells. Elevated levels of Cleaved-Poly (ADP-ribose) polymerase-1 (PARP1) and microRNA-155 (MiR-155) are related to poor prognosis of disease. Berberine as a natural isoquinoline alkaloid, has shown an anti-tumor potential in tumor cells. The objective of present study was to explore some aspects of molecular mechanisms of berberine effect in CLL cells. To analyze the expression of CD69 and Ki67 using flow cytometry, 16 peripheral blood samples and seven bone marrow aspirates were collected from CLL patients. Isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) were treated with 25 µM of berberine for 24 h. The level of miR-155 expression was subsequently evaluated by real-time PCR. Furthermore, western blot was used for assessment of cleaved PARP1. Our results demonstrated a significant reduction in CD69 and Ki67 expression on CD19 + cells when the cells were treated by berberine. Interestingly, the expression level of miR-155 was reduced after berberine treatment in compare to the control group. Furthermore, western blotting revealed an increased level of cleaved PARP1 in dose-dependently manner in CLL cells. The results confirmed the anti-tumor impact of berberine on CLL cells through reducing CD69, Ki67, and miR-155 expression and increasing cleaved PARP1 may be considered as an option for future clinical studies.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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