Simultaneous determination of vitamin B 6 and magnesium using capillary electrophoresis coupled with contactless conductivity detection: Method development, validation, and application to pharmaceutical and nutraceutical samples.

Autor: Huyen My Dang T; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam., Quoc Hoang A; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam. Electronic address: hoangquocanh1990@gmail.com., Huy Nguyen Q; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam; Faculty of Pharmacy, University of Medicine and Pharmacy, Thai Nguyen University, 284, Luong Ngoc Quyen, Thai Nguyen 24000, Vietnam., Chi Le D; Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi 10000, Vietnam., Duc Mai T; Université Paris-Saclay, CNRS, Institut Galien Paris-Saclay, 92296, Châtenay-Malabry, France., Nhi Do Y; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam., Anh Kieu V; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam., Khanh Cao C; National Institute for Food Control (NIFC), 65, Pham Than Duat, Hanoi 10000, Vietnam., Minh Hien Lu T; National Institute for Food Control (NIFC), 65, Pham Than Duat, Hanoi 10000, Vietnam., Minh Thu Nguyen T; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam., Pham B; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam., Anh Huong Nguyen T; Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19, Le Thanh Tong, Hanoi 10000, Vietnam. Electronic address: nguyenthianhhuong@hus.edu.vn.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2022 Nov 01; Vol. 1210, pp. 123471. Date of Electronic Publication: 2022 Sep 24.
DOI: 10.1016/j.jchromb.2022.123471
Abstrakt: Vitamins and minerals are usually incorporated in pharmaceutical and nutraceutical products, but a simple, rapid, and inexpensive analytical method for their simultaneous determination is still lacking. In this study, we developed a quantification method for pyridoxine (vitamin B 6 ) and magnesium (Mg) by using purpose-made capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C 4 D) instrument. Main analytical conditions include: fused silica capillary (total length 55 cm, effective length 40 cm, inner diameter 50 μm); background electrolyte consisted of 10 mM L-arginine/acetic acid (pH 5) with 20% acetonitrile; separation voltage + 20 kV; hydrodynamic injection (siphoning at 20 cm in 25 s). Detection limits of vitamin B 6 and Mg were 1 and 0.1 mg/L, respectively. Good linearity (R 2  > 0.999) was observed for vitamin B 6 and Mg calibration curves over concentration ranges of 3-100 and 0.3-200 mg/L, respectively. The method was applied to analyze vitamin B 6 and Mg in several pharmaceutical and nutraceutical samples. The analytical results obtained by our method were in good agreement with reference methods (i.e., HPLC for vitamin B 6 and ICP-OES for Mg). High-efficient and low-cost CE-C 4 D method can accordingly serve as a promising tool for concurrent analysis of inorganic and organic species in pharmaceutical and nutraceutical analysis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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