New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean.

Autor: Ballesteros-Contreras DC; Faculty of Science and Engineering, Manchester Metropolitan University-MMU, Manchester, United Kingdom.; Instituto de Investigaciones Marinas y Costeras de Colombia-INVEMAR, Santa Marta D.T.C.H., Colombia., Barrios LM; School of Biological and Marine Sciences, University of Plymouth, Plymouth, United Kingdom., Preziosi R; School of Biological and Marine Sciences, University of Plymouth, Plymouth, United Kingdom.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Sep 28; Vol. 17 (9), pp. e0274895. Date of Electronic Publication: 2022 Sep 28 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0274895
Abstrakt: Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation. Madracis auretenra in particular, is a widespread shallow coral in marine protected areas (MPAs) of the Caribbean. Due to the important role of MPAs as a biodiversity conservation tool, this species can be used as a model to estimate its dispersion/migration among Caribbean reefs through the use of informative genetic markers (microsatellites) specifically designed for it. Seventeen new polymorphic microsatellites markers for M. auretenra were developed and tested in 330 samples from Colombia, Guatemala, Curacao and Barbados. The gene diversity (1-D) ranged from 0.482 to 0.903, while the evenness ranged from 0.456 to 0.884 after clone removal. The expected heterozygosity (He) ranged from 0.347 to 0.742, while the observed heterozygosity (Ho) ranged from 0.078 to 0.578. The specificity of our microsatellites shows the potential use of these markers in a-posteriori analysis to detect population structure at different spatial scales, where M. auretenra is reported.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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