| Autor: |
Islam MT; Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.; Infectious Diseases Division, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR, B), Dhaka, Bangladesh., Liang K; Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada., Orata FD; Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.; Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada., Im MS; Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Alam M; Infectious Diseases Division, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR, B), Dhaka, Bangladesh., Lee CC; Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Boucher YF; Saw Swee Hock School of Public Health and National University Hospital System, National University of Singapore, Singapore.; Singapore Centre for Environmental Life Sciences Engineering, National University of Singapore, Singapore. |
| Abstrakt: |
A number of bacteria with close resemblance to Vibrio cholerae have been isolated over the years by the Centres for Disease Control and Prevention (CDC), which could not be assigned a proper taxonomic designation on the basis of the results from preliminary identification methods. Nine such isolates have been found to share 16S rRNA gene identity exceeding 99 % with V. cholerae, yet DNA-DNA hybridization (60.4-62.1 %) and average nucleotide identity values (94.4-95.1 %) were below the species cut-off, indicating a potentially novel species. Phylogenetic analysis of core genomes places this group of isolates in a monophyletic clade, within the 'Cholerae clade', but distinct from any other species. Extensive phenotypic characterization reveals unique biochemical properties that distinguish this novel species from V. cholerae . Comparative genomic analysis reveals a unique set of siderophore genes, indicating that iron acquisition strategies could be vital for the divergence of the novel species from a common ancestor with V. cholerae . On the basis of the genetic, phylogenetic and phenotypic differences observed, we propose that these isolates represent a novel species of the genus Vibrio , for which the name Vibrio tarriae sp. nov. is proposed. Strain 2521-89 T (= DSM 112461=CCUG 75318), isolated from lake water, is the type strain. |
