Activity of arginine-phenylalanine and arginine-tryptophan-based surfactants against Staphylococcus aureus .

Autor: Moreno LS; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Nascimento FB; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., da Silva CR; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Sá LG; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Faculty of Biomedicine, Unichristus University Center, Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Neto JB; Faculty of Biomedicine, Unichristus University Center, Fortaleza, Brazil., Silva J; Department of Chemistry, Group of Theoretical Chemistry and Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, Brazil., Silva EM; Department of Chemistry, Group of Theoretical Chemistry and Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, Brazil., Dos Santos HS; Department of Chemistry, Group of Theoretical Chemistry and Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, Brazil., Pérez L; Department of Surfactants & Nanobiotechnology, IQAC-CSIC, Barcelona, Spain., da Silva AR; Department of Surfactants & Nanobiotechnology, IQAC-CSIC, Barcelona, Spain., Rodrigues DS; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Barbosa AD; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Moreira LE; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Cavalcanti BC; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., de Morais MO; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil., Júnior HV; Department of Clinical & Toxicological Analyses, School of Pharmacy, Federal University of Ceará (UFC), Fortaleza, Brazil.; Drug Research & Development Center, Federal University of Ceará (UFC), Fortaleza, Brazil.
Jazyk: angličtina
Zdroj: Future microbiology [Future Microbiol] 2022 Nov; Vol. 17, pp. 1363-1379. Date of Electronic Publication: 2022 Sep 28.
DOI: 10.2217/fmb-2022-0074
Abstrakt: Aims: This study aimed to evaluate the antibacterial effect of two new cationic surfactants based on phenylalanine-arginine (LPAM) and tryptophan-arginine (LTAM). Materials & methods: Antibacterial activity, mechanism of action and interactions with Staphylococcus aureus enzymes were measured through microbiological, flow cytometry and molecular docking assays, respectively. Results & conclusion: These compounds showed antibacterial activity in the range of 4.06-16.24 μg/ml against planktonic cells and no activity against mature biofilms, since they caused a loss of membrane integrity and increased DNA damage, as revealed by flow cytometry analysis. In silico assays revealed the existence of molecular bonds such as hydrogen bonds, mainly with DNA. Therefore, these compounds have promising pharmacological activity against MRSA strains.
Databáze: MEDLINE