Assessment of Regional Lung Ventilation with Positron Emission Tomography Using the Radiofluorinated Gas [ 18 F]SF 6 : Application to an Animal Model of Impaired Ventilation.

Autor: Passannante R; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain., Gómez-Vallejo V; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain., Cossío U; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain., Ruiz-Cabello J; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain.; Ikerbasque, Basque Foundation for Science, Plaza Euskadi 5, 48009, Bilbao, Spain.; Centro de Investigación Biomédica en Red - Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, 28029, Madrid, Spain.; Departamento de Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense Madrid, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain., Lekuona A; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain., Salinas V; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain., Amado-Rodríguez L; Centro de Investigación Biomédica en Red - Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, 28029, Madrid, Spain.; Hospital Universitario Central de Asturias, Unidad de Cuidados Críticos Cardiológicos, 33011, Oviedo, Spain.; Instituto de Investigación Sanitaria del Principado de Asturias, 33011, Oviedo, Spain., Albaiceta GM; Centro de Investigación Biomédica en Red - Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, 28029, Madrid, Spain.; Hospital Universitario Central de Asturias, Unidad de Cuidados Críticos Cardiológicos, 33011, Oviedo, Spain.; Instituto de Investigación Sanitaria del Principado de Asturias, 33011, Oviedo, Spain.; Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33005, Oviedo, Spain., Martín A; Ikerbasque, Basque Foundation for Science, Plaza Euskadi 5, 48009, Bilbao, Spain.; Laboratory of Neuroimaging and Biomarkers of Inflammation, Achucarro Basque Center for Neuroscience, Science Park UPV/EHU, Sede Building B. Sarriena, 48940, Leioa, Spain., Rejc L; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia., Llop J; CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo Miramón 182, 20014, San Sebastian, Guipuzcoa, Spain. jllop@cicbiomagune.es.
Jazyk: angličtina
Zdroj: Molecular imaging and biology [Mol Imaging Biol] 2023 Apr; Vol. 25 (2), pp. 413-422. Date of Electronic Publication: 2022 Sep 27.
DOI: 10.1007/s11307-022-01773-7
Abstrakt: Purpose: Clinical ventilation studies are primarily performed with computerized tomography (CT) and more often with single-photon emission Computerized tomography (SPECT) using radiolabelled aerosols, both presenting certain limitations. Here, we investigate the use of the radiofluorinated gas [ 18 F]SF 6 as a positron emission tomography (PET) ventilation marker in an animal model of impaired lung ventilation.
Procedures: Sprague-Dawley rats (n = 15) were randomly assigned to spontaneous ventilation (sham group), endotracheal administration of phosphate-buffered saline (PBS group), or endotracheal administration of lipopolysaccharide (LPS group). PET-[ 18 F]SF 6 images (10-min acquisition) were acquired at t = 48 h after LPS or PBS administration under mechanical ventilation. CT images were acquired after each PET session. Volumes of interest were manually delineated in the lungs on CT images, and voxel-by-voxel analysis was carried out on PET images to obtain the corresponding histograms. After the imaging sessions, lungs were harvested to conduct histological analysis.
Results: Ventilation studies in sham animals showed uniform distribution of [ 18 F]SF 6 and fast elimination of the radioactivity after discontinuation of the administration. For PBS- and LPS-treated rats, ventilation defects were observed on PET images in some animals, identified as regions with low presence of the radiolabelled gas. Hypoventilated areas co-localized with regions with higher x-ray attenuation than healthy lungs on the CT images, suggesting the presence of oedema and, in some cases, atelectasis. Histograms obtained from PET images showed quasi-Gaussian distributions for control animals, while PBS- and LPS-treated animals demonstrated the presence of hypoventilated voxels. Deviation of the histograms from Gaussian distribution correlated with histological score was obtained by ex vivo histological analysis.
Conclusions: [ 18 F]SF 6 is an appropriate marker of regional lung ventilation and may find application in the early diagnose of acute lung disease.
(© 2022. The Author(s), under exclusive licence to World Molecular Imaging Society.)
Databáze: MEDLINE
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