The evaluation of citral effects on experimental unilateral testicular ischemia/reperfusion injury.

Autor: Rezakhaniha B; Department of Urology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran., Ganjiani V; Department of Urology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Siroosbakht S; Department of Urology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran., Ahmadi N; Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Meimandi-Parizi A; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Divar MR; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Shrifiyazdi H; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Mirghazanfari SM; Department of Basic Sciences, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2022 Dec; Vol. 54 (11), pp. e14605. Date of Electronic Publication: 2022 Sep 26.
DOI: 10.1111/and.14605
Abstrakt: This investigation aimed to evaluate the defensive impacts of citral on ischemia/reperfusion (I/R) injury induced by testicular torsion/detorsion (T/D) in rats in an experimental model. The grouping of subjects was as follows: (1) sham, (2) T/D, (3) and (4) T/D plus citral 150 and 300 mg/kg, respectively, and (5) intact (citral 300 mg/kg). T/D was performed by testicular 720° turning for 2 h and then detorsion for 24 h. Blood serum was obtained to assess testosterone and oxidative stress markers, epididymal sperms were collected for sperm staining and sperm analysis, and testicular tissues were examined for histopathology. T/D damage was associated with a remarkable decline in sperm total count, viability, and some velocity parameters in comparison to the sham group (p < 0.05), which could be reversed significantly by citral (p < 0.05). Histopathologically, T/D damage caused severe oedema, haemorrhage, inflammation, and seminiferous tubules disruption, while citral improved significantly the mean seminiferous tubular diameter, Cosentino's score, and Johnsen's score (p < 0.05). I/R injury was associated with significant increased malondialdehyde and oxidative stress index, and also significant reduced total antioxidant capacity and testosterone versus the sham group (p < 0.05), which all were prevented significantly by citral administration (p < 0.05). The outcomes greatly proved that testicular I/R injury could be significantly prevented by citral.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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