In vivo metabolic imaging identifies lipid vulnerability in a preclinical model of Her2+/Neu breast cancer residual disease and recurrence.

Autor: Madonna MC; Department of Biomedical Engineering, Duke University, Durham, NC, USA. megan.madonna@duke.edu., Duer JE; Duke University Trinity College of Arts and Sciences, Durham, NC, USA., McKinney BJ; Department of Pharmacology & Cancer Biology, School of Medicine, Duke University, Durham, NC, USA., Sunassee ED; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Crouch BT; Department of Biomedical Engineering, Duke University, Durham, NC, USA., Ilkayeva O; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Durham, NC, USA., Hirschey MD; Department of Pharmacology & Cancer Biology, School of Medicine, Duke University, Durham, NC, USA.; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Durham, NC, USA.; Department of Medicine, Division of Endocrinology, Metabolism, and Nutrition, Duke University Medical Center, Durham, NC, USA., Alvarez JV; Department of Pharmacology & Cancer Biology, School of Medicine, Duke University, Durham, NC, USA., Ramanujam N; Department of Biomedical Engineering, Duke University, Durham, NC, USA.; Department of Pharmacology & Cancer Biology, School of Medicine, Duke University, Durham, NC, USA.
Jazyk: angličtina
Zdroj: NPJ breast cancer [NPJ Breast Cancer] 2022 Sep 26; Vol. 8 (1), pp. 111. Date of Electronic Publication: 2022 Sep 26.
DOI: 10.1038/s41523-022-00481-3
Abstrakt: Recurrent cancer cells that evade therapy is a leading cause of death in breast cancer patients. This risk is high for women showing an overexpression of human epidermal growth factor receptor 2 (Her2). Cells that persist can rely on different substrates for energy production relative to their primary tumor counterpart. Here, we characterize metabolic reprogramming related to tumor dormancy and recurrence in a doxycycline-induced Her2+/Neu model of breast cancer with varying times to recurrence using longitudinal fluorescence microscopy. Glucose uptake (2-NBDG) and mitochondrial membrane potential (TMRE) imaging metabolically phenotype mammary tumors as they transition to regression, dormancy, and recurrence. "Fast-recurrence" tumors (time to recurrence ~55 days), transition from glycolysis to mitochondrial metabolism during regression and this persists upon recurrence. "Slow-recurrence" tumors (time to recurrence ~100 days) rely on both glycolysis and mitochondrial metabolism during recurrence. The increase in mitochondrial activity in fast-recurrence tumors is attributed to a switch from glucose to fatty acids as the primary energy source for mitochondrial metabolism. Consequently, when fast-recurrence tumors receive treatment with a fatty acid inhibitor, Etomoxir, tumors report an increase in glucose uptake and lipid synthesis during regression. Treatment with Etomoxir ultimately prolongs survival. We show that metabolic reprogramming reports on tumor recurrence characteristics, particularly at time points that are essential for actionable targets. The temporal characteristics of metabolic reprogramming will be critical in determining the use of an appropriate timing for potential therapies; namely, the notion that metabolic-targeted inhibition during regression reports long-term therapeutic benefit.
(© 2022. The Author(s).)
Databáze: MEDLINE
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