The therapeutical effects of damage-specific stress induced exosomes on the cisplatin nephrotoxicity IN VIVO.

Autor: Oz Oyar E; Department of Physiology, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey. Electronic address: eser.oz.oyar@ikc.edu.tr., Aciksari A; Department of Stem Cell, Institute of Health Sciences, Kocaeli University, Izmit, Kocaeli, Turkey. Electronic address: aysegul.aciksari@kocaeli.edu.tr., Azak Pazarlar B; Department of Physiology, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey. Electronic address: burcu.azak@ikc.edu.tr., Egilmez CB; Department of Physiology, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey. Electronic address: cansu.bilister@ikc.edu.tr., Duruksu G; Department of Stem Cell, Institute of Health Sciences, Kocaeli University, Izmit, Kocaeli, Turkey; Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Izmit, Kocaeli, Turkey. Electronic address: gokhan.duruksu@kocaeli.edu.tr., Rencber SF; Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, Izmit, Kocaeli, Turkey. Electronic address: selenay.furat@kocaeli.edu.tr., Yardimoglu Yilmaz M; Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, Izmit, Kocaeli, Turkey. Electronic address: melda@kocaeli.edu.tr., Ozturk A; Department of Stem Cell, Institute of Health Sciences, Kocaeli University, Izmit, Kocaeli, Turkey. Electronic address: ahmet.ozturk@kocaeli.edu.tr., Yazir Y; Department of Stem Cell, Institute of Health Sciences, Kocaeli University, Izmit, Kocaeli, Turkey; Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Izmit, Kocaeli, Turkey; Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, Izmit, Kocaeli, Turkey. Electronic address: yusufhan.yazir@kocaeli.edu.tr.
Jazyk: angličtina
Zdroj: Molecular and cellular probes [Mol Cell Probes] 2022 Dec; Vol. 66, pp. 101861. Date of Electronic Publication: 2022 Sep 24.
DOI: 10.1016/j.mcp.2022.101861
Abstrakt: Cisplatin is one of the metal containing drugs for the solid cancer treatments. However, its side-effects limit its application in the cancer treatment. Stem cell therapy is a promising treatment for the tissue damage caused by the chemotherapeutic agents, like cisplatin. Exosomes secreted by mesenchymal stem cells (MSCs) could be used for cell-free regenerative treatment, but their potency and reproducibility are questionable. In this study, the microenvironment of the renal tubular epithelial cells was mimicked by coculture of endothelial-, renal proximal tubule epithelial- and fibroblast cells. Cisplatin was applied to this tricell culture model, and the secreted rescue signals were collected and used to induce MSCs. From these stress-induced MSCs, the (stress-induced) exosomes were collected and used for the cell-free therapeutic treatment of cisplatin-treated rats with acute kidney injury. The composition of the stress-induces exosomes was compared with the non-induced exosomes and found that the expression of some critical factors for cell proliferation, repair mechanism and oxidative stress was improved. The cisplatin-damaged renal tissue showed substantial recovery after the treatment with stress-induced exosomes compared to the treatment with non-induced exosomes. Although, the non-induced exosomes showed their activity mostly as cytoprotective, the induced exosomes further involved actively in the tissue regeneration, like MSCs. It was shown that the exosomes could be reprogrammed to improve their therapeutic effect to be used in cell-free regenerative medicine. Further, cisplatin-induced tissue damage in the kidney might be effectively prevented and used for tissue regeneration by use of induced exosomes generated for a particular damage.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2022. Published by Elsevier Ltd.)
Databáze: MEDLINE
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