Interaction of negatively and positively capped gold nanoparticle with different lipid model membranes.

Autor: Sheridan AJ; Department of Biological Sciences and Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK. Electronic address: a.sheridan@mail.cryst.bbk.ac.uk., Thompson KC; Department of Biological Sciences and Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK. Electronic address: k.thompson@mail.cryst.bbk.ac.uk., Slater JM; Department of Biological Sciences and Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK.
Jazyk: angličtina
Zdroj: Biophysical chemistry [Biophys Chem] 2022 Nov; Vol. 290, pp. 106896. Date of Electronic Publication: 2022 Sep 19.
DOI: 10.1016/j.bpc.2022.106896
Abstrakt: The use of functionalised gold nanoparticles in biomedical applications is expanding. Here we explore the interaction of gold nanoparticles with lipid membranes using readily available equipment and basic techniques to explore how the charge on the nanoparticles, and the nature of the lipid, influences the interaction. Gold nanoparticles were synthesised with two different surface functionalisations, negatively charged citrate groups and positively charged cetyltrimethylammonium groups from CTAB, to determine how surface charge affects the interaction of the nanoparticles with the Zwitterionic lipid POPC and the anionic lipid POPG. It was observed that the surface pressure/area isotherms of POPG monolayers on exposure to citrate capped nanoparticles were not shifted to higher molecular areas as much as those of POPC, suggesting that the anionic headgroups of the POPG lipid repel the anionic surface charge of the citrate capped nanoparticles to some extent limiting inclusion. In contrast, the surface pressure/area isotherms of the POPG monolayers exposed to CTAB capped nanoparticles are shifted to higher molecular areas more than for the POPC monolayers. The interaction of anionic nanoparticles with lipid bilayers was measured by the mass change of the bilayer deposited on the surface of a quartz crystal microbalance (QCM) exposed to nanoparticles in an aqueous phase flow. The QCM frequency changes show that bilayers of unsaturated phosophocholine lipids readily took up particles, whereas for the saturated lipid DPPC significant uptake was only observed when the bilayer was warmed to above its gel-to-fluid transition temperature, T m. This is possibly due to an increase in the molecular mobility and bilayer bending modulus, κ, of the bilayer.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: