Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon.

Autor: Betouke Ongwe ME; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut de Recherches en Écologie Tropicale, Centre National de la Recherche Scientifique et Technologique (CENAREST), Lambaréné, Gabon., Mouwenda YD; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon., Stam KA; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Kremsner PG; Institut für Tropenmedizin, Eberhad Karls Universität Tübingen, Tübingen, Germany., Lell B; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Division of Infectious Diseases and Tropical Medicine, Department of Medicine I, Medical University of Vienna, Vienna, Austria., Diemert D; George Washington University, Washington, DC, United States of America., Bethony J; George Washington University, Washington, DC, United States of America., Bottazzi ME; Texas Children's Hospital Center for Vaccine Development, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, United States of America., Hotez PJ; Texas Children's Hospital Center for Vaccine Development, National School of Tropical Medicine, Baylor College of Medicine, Houston, TX, United States of America., Leeuwen RV; Amsterdam Institute for Global Health and Development, Amsterdam, The Netherlands., Grobusch MP; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Eberhad Karls Universität Tübingen, Tübingen, Germany.; Department of Infectious Diseases, Center of Tropical Medicine and Travel Medicine, Amsterdam University Medical Center, Amsterdam Public Health; Amsterdam Infection & Immunity, University of Amsterdam, Amsterdam, The Netherlands., Adegnika AA; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Eberhad Karls Universität Tübingen, Tübingen, Germany.; German Center for Infection Research (DZIF), Tübingen, Germany., Mayboroda OA; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands., Yazdanbakhsh M; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Sep 26; Vol. 17 (9), pp. e0275013. Date of Electronic Publication: 2022 Sep 26 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0275013
Abstrakt: Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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