Protein Crystallization of Two Recombinant Lpt Proteins.
| Autor: | Bollati M; Department of Biosciences, Università degli Studi di Milano, Milan, Italy.; Institute of Biophysics - CNR, Milan, Italy., Gourlay LJ; Department of Biosciences, Università degli Studi di Milano, Milan, Italy. louise.gourlay@unimi.it. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2548, pp. 249-263. |
| DOI: | 10.1007/978-1-0716-2581-1_15 |
| Abstrakt: | The prerequisite for 3D structure determination of macromolecules via X-ray crystallography is well-ordered, diffracting crystals. Here, we report the recombinant production, biophysical/biochemical protein sample characterization, and vapor diffusion sitting drop crystallization protocols for two lipopolysaccharide transport proteins: LptH from Pseudomonas aeruginosa (Pa-LptH) and an inactive LptC mutant (G153R) from Escherichia coli (EcLptC (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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