| Autor: |
Patrocinio TA; UNIFENAS. Rodovia MG 179 - Km 0. C.P. 37130-000, Minas Gerais, Brazil., Fernandes CAC; UNIFENAS. Rodovia MG 179 - Km 0. C.P. 37130-000, Minas Gerais, Brazil.; BIOTRAN, Rua Tatuin 447, C.P. 37130-000, MG, Brazil., Macedo GC; Universidade Federal de Juiz de Fora, UFJF, Rua José Lourenço Kelmer, 36036-900, MG, Brazil., Silva ADD; Universidade Federal de Juiz de Fora, UFJF, Rua José Lourenço Kelmer, 36036-900, MG, Brazil., Barbosa NRR; Universidade Federal de Juiz de Fora, UFJF, Rua José Lourenço Kelmer, 36036-900, MG, Brazil., Quintao CCR; Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de Leite, C.P. 36038-330, MG, Brazil., Ribeiro MEDS; UNIFENAS. Rodovia MG 179 - Km 0. C.P. 37130-000, Minas Gerais, Brazil., Camargo LSA; UNIFENAS. Rodovia MG 179 - Km 0. C.P. 37130-000, Minas Gerais, Brazil.; Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de Leite, C.P. 36038-330, MG, Brazil. |
| Abstrakt: |
Oxidative stress is an undesirable effect of in vitro culture, which requires antioxidant supplementation. This study investigated the analogue of resveratrol (RA33) as an alternative to resveratrol, an antioxidant molecule, for the in vitro culture of in vitro -fertilized bovine embryos. The effect of different concentrations of RA33 on embryo development was evaluated and a comparison between RA33 and resveratrol was performed. The cleavage rate was higher ( P < 0.05) with 2.5 μM (69.0 ± 4.4%) than at 0, 0.1 or 0.5 μM RA33 (62.1 ± 2.0%, 60.7 ± 5.9% and 56.7 ± 5.8%, respectively). The blastocyst rates on days 7 and 8 post-fertilization with 2.5 μM RA33 (19.4 ± 3.3% and 24.6 ± 3.3%, respectively) were higher ( P < 0.05) than for 0 μM (12.4 ± 2.5% and 15.2±2.5%, respectively). When 2.5 μM RA33 was compared with 0.5 μM resveratrol, similar ( P > 0.05) cleavage and blastocyst rates were found between them, but the cleavage rate was higher ( P < 0.05) in the control (80.8 ± 3.4%) than for the resveratrol treatment (76.4 ± 3.6%). The numbers of apoptotic cells and the apoptotic index were lower ( P < 0.05) with RA33 (6.5 ± 0.6 cells and 6.4 ± 0.7%, respectively) and resveratrol (5 ± 0.8 cells and 5.5 ± 1.0%, respectively) than in the control group (9.8 ± 1.2 cells and 8.9 ± 1.1%, respectively). In conclusion, RA33 can enhance the preimplantation development of in vitro -fertilized bovine embryos and be an alternative to resveratrol in embryo culture medium. |
