Cytotoxic cycloartane triterpenoid saponins from Actaea vaginata and their mechanism of action.

Autor: Li X; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Zhao Z; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Sun Z; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Sun Z; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Ma G; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Zhao X; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Xu X; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Yang M; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China., Wu X; Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province and Key Laboratory of Hunan Higher Education for Western Hunan Medicinal Plant and Ethnobotany, Huaihua University, Huaihua, 418008, China., Wu H; Beijing Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China; Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province and Key Laboratory of Hunan Higher Education for Western Hunan Medicinal Plant and Ethnobotany, Huaihua University, Huaihua, 418008, China. Electronic address: hfwu@implad.ac.cn., Zou Q; Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province and Key Laboratory of Hunan Higher Education for Western Hunan Medicinal Plant and Ethnobotany, Huaihua University, Huaihua, 418008, China. Electronic address: zouqioyu@126.com., Zhang J; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultura Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian, 223300, China. Electronic address: zhangji@hytc.edu.cn.
Jazyk: angličtina
Zdroj: Carbohydrate research [Carbohydr Res] 2022 Nov; Vol. 521, pp. 108673. Date of Electronic Publication: 2022 Sep 08.
DOI: 10.1016/j.carres.2022.108673
Abstrakt: A further phytochemical investigation of the whole plants of Actaea vaginata afforded two new cycloartane triterpenoid saponins, (20S*,24R*)-15α,16β-diacetoxy-20,24-epoxy-9,19-cyclolanostane-3β,25-diol-3-O-β-d-xylopyranoside (1) and (20S)-15β,16β -diacetoxy-18,20-epoxy-3β,25-diol-24-oxo-9,19-cyclolanostan-3-O-β-D-xylo-pyrano-syl-25-O-β-d-glucopyranoside (2), together with four known compounds (3-6). Their structures were established on the basis of extensive analysis of NMR and HRESIMS data as well as by comparison with the reported data in the literature. All the isolates were evaluated for their cytotoxic activities against human hepatocellular carcinoma HepG2 cell line. Compounds 1 and 2 exhibited weak cytotoxicity with IC 50 values of 36.10 and 27.39 μM, respectively. In addition, beesioside I (6) was found to significantly inhibit proliferation and induce apoptosis in HepG2 cells. A closer examination of underlying mechanism revealed that beesioside I could increase the levels of ROS and caspase-3 and promote phosphorylation of JNK in the JNK signaling pathway. Molecular modeling studies also shed further light on how beesioside I interacted with the key protein kinase.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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