| Autor: |
Brnić D; Virology Department, Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia., Čolić D; Virology Department, Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia.; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac 10, 08028 Barcelona, Spain., Kunić V; Virology Department, Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia., Maltar-Strmečki N; Laboratory for Electron Spin Spectroscopy, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia., Krešić N; Virology Department, Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia., Konjević D; Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia., Bujanić M; Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia., Bačani I; Animal Feed Factory Ltd., Dr. Ivana Novaka 11, 40000 Čakovec, Croatia., Hižman D; Belje Agro-Vet plus Ltd., Kokingrad 4, Mece, 31326 Darda, Croatia.; Rusagro, LLC 'Tambovsky bacon', Bazarnaya 104, 392036 Tambov, Russia., Jemeršić L; Virology Department, Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, Croatia. |
| Abstrakt: |
Rotavirus A (RVA) is an important pathogen for porcine health. In comparison to humans, RVA in domestic animals and especially in wildlife is under researched. Therefore, the aim of the present study was to investigate the prevalence, genetic diversity, molecular epidemiology and interspecies transmission of RVA in domestic pigs and wild boars. During the three consecutive RVA seasons (2018-2021) we collected 445 and 441 samples from domestic pigs and wild boars, respectively. Samples were tested by real-time RT-PCR, and RVA-positive samples were genotyped in VP7 and VP4 segments. Our results report an RVA prevalence of 49.9% in domestic pigs and 9.3% in wild boars. Outstanding RVA genetic diversity was observed in VP7 and VP4 segments, especially in domestic pigs exhibiting a striking 23 different RVA combinations (G5P[13] and G9P[23] prevailed). Interspecies transmission events were numerous between domestic pigs and wild boars, sharing G3, G5, G6, G9, G11 and P[13] genotypes. Furthermore, our data indicate that such transmission events involved even bovines (G6, P[11]) and, intriguingly, humans (G1P[8]). This study contributes to the basic knowledge that may be considered important for vaccine development and introduction, as a valuable and currently missing tool for efficient pig health management in the EU. |
