| Autor: |
Lira-Amaya JJ; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico., Santamaria-Espinosa RM; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico., Castañeda-Arriola RO; Sitio Experimental Pichucalco, CIRGOC, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Km. 8, Carretera Pichucalco-Teapa, Pichucalco 86040, Mexico., Martínez-García G; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico., Polanco-Martínez DJ; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico., Rojas-Martínez C; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico., Alvarez-Martínez JÁ; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico., Figueroa-Millán JV; CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec 62550, Mexico. |
| Abstrakt: |
Two hundred and thirty-three blood samples of water buffalo were collected on four farms in Veracruz state and Tabasco state, Mexico, to detect and confirm the identities of Babesia and Anaplasma spp. sequences. Nested PCR assays were used for the amplification of specific genes encoding B. bovis rhoptry-associated protein (RAP-1), B. bigemina Spe I- Ava I restriction fragment, and Anaplasma marginale major surface protein 5 (MSP5). Using DNA sequencing and BLASTn analysis for DNA homology hemoparasite identification, the identities of the hemoparasites were established by comparing the nucleotide sequences obtained in this study with those available in the GenBank database at the National Center for Biotechnology Information (NCBI). Water buffalo infection with at least one of the hemoparasites under study was detected in 45% (105/233) of the blood samples, while a mixed infection with B. bovis and B. bigemina was detected in 6.4% (15/233) of samples. For this cross-sectional study, mixed infections with the three hemoparasites were not detected. BLASTn analysis revealed that the nucleotide sequences of the water buffalo isolates shared sequence identity values ranging from 88 to 100% with previously published gene sequences of B. bovis , B. bigemina , and A. marginale . The current results confirm that water buffalo, as cattle, are also carriers of hemoparasite infections that are tick-transmitted, and suggest that they probably have an important role in the epidemiology of bovine babesiosis in Mexico. |
