Monitoring Therapeutic Responses to Silicified Cancer Cell Immunotherapy Using PET/MRI in a Mouse Model of Disseminated Ovarian Cancer.

Autor: Taylor EN; Department of Radiology, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Wilson CM; Department of Radiology, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Franco S; Internal Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., De May H; Department of Obstetrics & Gynecology, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Medina LY; Internal Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Yang Y; Pharmaceutical Sciences, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Flores EB; Internal Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Bartee E; Internal Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Selwyn RG; Department of Radiology, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA., Serda RE; Internal Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Sep 10; Vol. 23 (18). Date of Electronic Publication: 2022 Sep 10.
DOI: 10.3390/ijms231810525
Abstrakt: Current imaging approaches used to monitor tumor progression can lack the ability to distinguish true progression from pseudoprogression. Simultaneous metabolic 2-deoxy-2-[ 18 F]fluoro-D-glucose ([ 18 F]FDG) positron emission tomography (PET) and magnetic resonance imaging (MRI) offers new opportunities to overcome this challenge by refining tumor identification and monitoring therapeutic responses to cancer immunotherapy. In the current work, spatial and quantitative analysis of tumor burden were performed using simultaneous [ 18 F]FDG-PET/MRI to monitor therapeutic responses to a novel silicified cancer cell immunotherapy in a mouse model of disseminated serous epithelial ovarian cancer. Tumor progression was validated by bioluminescence imaging of luciferase expressing tumor cells, flow cytometric analysis of immune cells in the tumor microenvironment, and histopathology. While PET demonstrated the presence of metabolically active cancer cells through [ 18 F]FDG uptake, MRI confirmed cancer-related accumulation of ascites and tissue anatomy. This approach provides complementary information on disease status without a confounding signal from treatment-induced inflammation. This work provides a possible roadmap to facilitate accurate monitoring of therapeutic responses to cancer immunotherapies.
Databáze: MEDLINE
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