A Transformation and Genome Editing System for Cassava Cultivar SC8.

Autor:	Wang YJ; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China., Lu XH; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China.; School of Life Sciences, Hainan University, Haikou 570228, China., Zhen XH; School of Life Sciences, Hainan University, Haikou 570228, China.; San Yan Research Institute, Chinese Academy of Tropical Agricultural Sciences & Hainan Yazhou Bay Seed Lab, Sanya 572025, China., Yang H; School of Life Sciences, Hainan University, Haikou 570228, China., Che YN; School of Life Sciences, Hainan University, Haikou 570228, China., Hou JY; School of Life Sciences, Hainan University, Haikou 570228, China., Geng MT; School of Life Sciences, Hainan University, Haikou 570228, China., Liu J; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China., Hu XW; School of Life Sciences, Hainan University, Haikou 570228, China., Li RM; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China., Guo JC; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China., Yao Y; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.; San Yan Research Institute, Chinese Academy of Tropical Agricultural Sciences & Hainan Yazhou Bay Seed Lab, Sanya 572025, China.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2022 Sep 14; Vol. 13 (9). Date of Electronic Publication: 2022 Sep 14.
DOI:	10.3390/genes13091650
Abstrakt:	Cassava starch is a widely used raw material for industrial production. South Chinese cassava cultivar 8 ( Manihot esculenta Crantz cv. SC8) is one of the main locally planted cultivars. In this study, an efficient transformation system for cassava SC8 mediated with Agrobacterium strain LBA4404 was presented for the first time. Cassava friable embryogenic calli (FECs) were transformed through the binary vector pCAMBIA1304 harboring GUS- and GFP- fused genes driven by the CaMV35S promoter. The transformation efficiency was increased in the conditions of Agrobacterium strain cell infection density (OD 600 = 0.65), 250 µM acetosyringone induction, and agro -cultivation with wet FECs for 3 days in dark. Based on the optimized transformation protocol, approximately 120-140 independent transgenic lines per mL settled cell volume (SCV) of FECs were created by gene transformation in approximately 5 months, and 45.83% homozygous mono-allelic mutations of the MePDS gene with a YAO promoter-driven CRISPR/Cas9 system were generated. This study will open a more functional avenue for the genetic improvement of cassava SC8.
Databáze:	MEDLINE
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