| Autor: |
Kruit SA; BIOS Lab on a Chip Group, MESA+ & TechMed Institutes, Max Planck Center for Complex Fluid Dynamics, University of Twente, 7500 AE Enschede, The Netherlands., de Bruijn DS; BIOS Lab on a Chip Group, MESA+ & TechMed Institutes, Max Planck Center for Complex Fluid Dynamics, University of Twente, 7500 AE Enschede, The Netherlands., Broekhuijse MLWJ; CRV Holding BV, 6843 NW Arnhem, The Netherlands.; Topigs Norsvin Research Center BV, 6641 SZ Beuningen, The Netherlands., Olthuis W; BIOS Lab on a Chip Group, MESA+ & TechMed Institutes, Max Planck Center for Complex Fluid Dynamics, University of Twente, 7500 AE Enschede, The Netherlands., Segerink LI; BIOS Lab on a Chip Group, MESA+ & TechMed Institutes, Max Planck Center for Complex Fluid Dynamics, University of Twente, 7500 AE Enschede, The Netherlands. |
| Abstrakt: |
Microfluidics and lab-on-chip technologies have been used in a wide range of biomedical applications. They are known as versatile, rapid, and low-cost alternatives for expensive equipment and time-intensive processing. The veterinary industry and human fertility clinics could greatly benefit from label-free and standardized methods for semen analysis. We developed a tool to determine the acrosome integrity of spermatozoa using microfluidic impedance cytometry. Spermatozoa from boars were treated with the calcium ionophore A23187 to induce acrosome reaction. The magnitude, phase and opacity of individual treated and non-treated (control) spermatozoa were analyzed and compared to conventional staining for acrosome integrity. The results show that the opacity at 19 MHz over 0.5 MHz is associated with acrosome integrity with a cut-off threshold at 0.86 (sensitivity 98%, specificity 97%). In short, we have demonstrated that acrosome integrity can be determined using opacity, illustrating that microfluidic impedance cytometers have the potential to become a versatile and efficient alternative in semen analysis and for fertility treatments in the veterinary industry and human fertility clinics. |
