| Autor: |
Moccia F; Department of Chemical Sciences, University of Naples 'Federico II', Via Cintia 4, I-80126 Naples, Italy., Liberti D; Department of Chemical Sciences, University of Naples 'Federico II', Via Cintia 4, I-80126 Naples, Italy., Giovando S; Centro Ricerche per la Chimica Fine Srl for Silvateam Spa, Via Torre 7, I-12080 San Michele Mondovì, Italy., Caddeo C; Department of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, I-09124 Cagliari, Italy., Monti DM; Department of Chemical Sciences, University of Naples 'Federico II', Via Cintia 4, I-80126 Naples, Italy., Panzella L; Department of Chemical Sciences, University of Naples 'Federico II', Via Cintia 4, I-80126 Naples, Italy., Napolitano A; Department of Chemical Sciences, University of Naples 'Federico II', Via Cintia 4, I-80126 Naples, Italy. |
| Abstrakt: |
Ellagic acid (EA) has long been recognized as a very active antioxidant, anti-inflammatory, and antimicrobial agent. However, its low bioavailability has often hampered its applications in health-related fields. Here, we report a phospholipid vesicle-based controlled release system for EA, involving the exploitation of chestnut wood mud (CWM), an industrial by-product from chestnut tannin production, as a largely available and low-cost source of this compound. Two kinds of CWM with different particle size distributions, indicated as CWM-A and CWM-B (<100 and 32 µm, respectively), containing 5 ± 1% w/w EA, were incorporated into transfersomes. The latter were small in size (~100 nm), homogeneously dispersed, and negatively charged. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assays indicated up to three-fold improvement in the antioxidant properties of CWM upon incorporation into transfersomes. The kinetics of EA released under simulated physiological conditions were evaluated by UV-Vis spectroscopy and HPLC analysis. The best results were obtained with CWM-B (100% of EA gradually released after 37 days at pH 7.4). A stepwise increase in the antioxidant properties of the released material was also observed. Cell-based experiments confirmed the efficacy of CWM-B transfersomes as antioxidant agents in contrasting photodamage. |
