A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System.

Autor: Ortega FG; IBS Granada, Instituto de Investigación Biosanitaria de Granada, 18012 Granada, Spain.; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustración 114, 18016 Granada, Spain., Gómez GE; Instituto de Investigaciones en Tecnología Química (INTEQUI), Departamento de Química, Universidad Nacional de San Luis, CONICET, Chacabuco y Pedernera, San Luis D5700BWS, Argentina., González-Martinez C; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustración 114, 18016 Granada, Spain., Valero T; IBS Granada, Instituto de Investigación Biosanitaria de Granada, 18012 Granada, Spain.; Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Campus Cartuja s/n, 18071 Granada, Spain., Expósito-Hernández J; IBS Granada, Instituto de Investigación Biosanitaria de Granada, 18012 Granada, Spain., Puche I; IBS Granada, Instituto de Investigación Biosanitaria de Granada, 18012 Granada, Spain., Rodriguez-Martinez A; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustración 114, 18016 Granada, Spain., Serrano MJ; IBS Granada, Instituto de Investigación Biosanitaria de Granada, 18012 Granada, Spain.; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustración 114, 18016 Granada, Spain.; Integral Oncology Division, Virgen de las Nieves University Hospital, 18071 Granada, Spain.; Department of Pathological Anatomy, School of Medicine, University of Granada, 18071 Granada, Spain., Lorente JA; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustración 114, 18016 Granada, Spain.; Lab. of Genetic Identification, Department of Legal Medicine & Toxicology and Physical Anthropology, School of Medicine, University of Granada, 18016 Granada, Spain., Fernández-Baldo MA; INQUISAL, Departamento de Química, Universidad Nacional de San Luis, CONICET, Chacabuco 917, San Luis D5700BWS, Argentina.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2022 Sep 16; Vol. 14 (18). Date of Electronic Publication: 2022 Sep 16.
DOI: 10.3390/cancers14184483
Abstrakt: We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at -100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL -1 to 1500 pg mL -1 . The sensitivity was demonstrated by a detection limit of 2 pg mL -1 and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.
Databáze: MEDLINE
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