Protein phosphorylation profile of Atlantic cod (Gadus morhua) in response to pre-slaughter pumping stress and postmortem time.

Autor: Zhang L; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; Department of Food Science, University of Copenhagen, 1958 Frederiksberg C, Denmark., Li Q; Department of Food Science, University of Copenhagen, 1958 Frederiksberg C, Denmark., Sørensen JS; National Food Institute, Technical University of Denmark, 2800 Kgs, Lyngby, Denmark., Luo Y; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. Electronic address: luoyongkang@263.net., Lametsch R; Department of Food Science, University of Copenhagen, 1958 Frederiksberg C, Denmark. Electronic address: rla@food.ku.dk.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2023 Feb 15; Vol. 402, pp. 134234. Date of Electronic Publication: 2022 Sep 13.
DOI: 10.1016/j.foodchem.2022.134234
Abstrakt: This study aimed to evaluate the effect of pumping stress (pumping and pumping-resting) and postmortem time (before and after rigor mortis) on phosphorylation profiles of myofibrillar protein (MP) and sarcoplasmic protein (SP) of Atlantic cod (Gadus morhua) fillets. The result showed that MP had higher global phosphorylation levels than SP regardless of stress condition and postmortem time. The pumping process resulted in significant changes in phosphorylation of structural proteins including myosin heavy and light chains. Pumping also affected the phosphorylation status of heat shock proteins and metabolic enzymes involved in the glycolytic pathways, indicating the possible role of phosphorylation in regulating energy hemostasis of fish under stressful conditions. The pumping-induced phosphorylation changes mainly occurred before rigor mortis, and postmortem time affected the phosphorylation status to a less extent. This work contributes to a deeper understanding on protein phosphorylation affected by pre-slaughter stress and postmortem time of fish.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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