| Autor: |
Ko SH; Department of Microbiology, College of Medicine, Gachon University, Incheon 21999, Korea.; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Korea., Lim Y; Department of Microbiology, College of Medicine, Gachon University, Incheon 21999, Korea.; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Korea., Kim EJ; Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Korea., Ko YW; Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Korea., Hong IS; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Korea.; Department of Molecular Medicine, College of Medicine, Gachon University, Incheon 21999, Korea.; Department of Health Science and Technology, Gachon Advanced Institute for Health Science & Technology, Gachon University, Incheon 21999, Korea., Kim S; Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Korea., Jung Y; Department of Microbiology, College of Medicine, Gachon University, Incheon 21999, Korea.; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Korea.; Department of Health Science and Technology, Gachon Advanced Institute for Health Science & Technology, Gachon University, Incheon 21999, Korea. |
| Abstrakt: |
The intestine and skin provide crucial protection against the external environment. Strengthening the epithelial barrier function of these organs is critical for maintaining homeostasis against inflammatory stimuli. Recent studies suggest that polar marine algae are a promising bioactive resource because of their adaptation to extreme environments. To investigate the bioactive properties of polar marine algae on epithelial cells of the intestine and skin, we created extracts of the Antarctic macroalgae Himantothallus grandifolius , Plocamium cartilagineum , Phaeurus antarcticus , and Kallymenia antarctica , analyzed the compound profiles of the extracts using gas chromatography-mass spectrometry, and tested the protective activities of the extracts on human intestinal and keratinocyte cell lines by measuring cell viability and reactive oxygen species scavenging. In addition, we assessed immune responses modulated by the extracts by real-time polymerase chain reaction, and we monitored the barrier-protective activities of the extracts on intestinal and keratinocyte cell lines by measuring transepithelial electrical resistance and fluorescence-labeled dextran flux, respectively. We identified bioactive compounds, including several fatty acids and lipid compounds, in the extracts, and found that the extracts perform antioxidant activities that remove intracellular reactive oxygen species and scavenge specific radicals. Furthermore, the Antarctic marine algae extracts increased cell viability, protected cells against inflammatory stimulation, and increased the barrier integrity of cells damaged by lipopolysaccharide or ultraviolet radiation. These results suggest that Antarctic marine algae have optimized their composition for polar environments, and furthermore, that the bioactive properties of compounds produced by Antarctic marine algae can potentially be used to develop therapeutics to promote the protective barrier function of the intestine and skin. |
