Resolving the internal morphology of core-shell microgels with super-resolution fluorescence microscopy.

Autor: Otto P; Physical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de., Bergmann S; Biomolecular Photonics, Bielefeld University Germany thomas.huser@physik.uni-bielefeld.de., Sandmeyer A; Biomolecular Photonics, Bielefeld University Germany thomas.huser@physik.uni-bielefeld.de., Dirksen M; Physical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de., Wrede O; Physical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de., Hellweg T; Physical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de., Huser T; Biomolecular Photonics, Bielefeld University Germany thomas.huser@physik.uni-bielefeld.de.
Jazyk: angličtina
Zdroj: Nanoscale advances [Nanoscale Adv] 2019 Nov 28; Vol. 2 (1), pp. 323-331. Date of Electronic Publication: 2019 Nov 28 (Print Publication: 2020).
DOI: 10.1039/c9na00670b
Abstrakt: We investigate the internal morphology of smart core-shell microgels by super-resolution fluorescence microscopy exploiting a combination of 3D single molecule localization and structured illumination microscopy utilizing freely diffusing fluorescent dyes. This approach does not require any direct chemical labeling and does not perturb the network structure of these colloidal gels. Hence, it allows us to study the morphology of the particles with very high precision. We found that the structure of the core-forming seed particles is drastically changed by the second synthesis step necessary for making the shell, resulting in a core region with highly increased dye localization density. The present work shows that super-resolution microscopy has great potential with respect to the study of soft colloidal systems.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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