Risk assessment on-a-chip: a cell-based microfluidic device for immunotoxicity screening.

Autor:	Oddo A; Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Victoria 3052 Australia nicolas.voelcker@monash.edu tommy.tong@monash.edu.; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Clayton Victoria 3168 Australia., Morozesk M; Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Victoria 3052 Australia nicolas.voelcker@monash.edu tommy.tong@monash.edu.; Universidade Federal de São Carlos, Departamento de Ciências Fisiológicas Rod. Washington Luiz, Km 235, São Carlos 13565-905 São Paulo Brazil., Lombi E; Future Industries Institute and UniSA STEM, University of South Australia Mawson Lakes 5095 South Australia Australia., Schmidt TB; Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Victoria 3052 Australia nicolas.voelcker@monash.edu tommy.tong@monash.edu.; Department of Applied Chemistry, Reutlingen University Alteburgstraße 150 72762 Reutlingen Germany., Tong Z; Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Victoria 3052 Australia nicolas.voelcker@monash.edu tommy.tong@monash.edu.; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Clayton Victoria 3168 Australia., Voelcker NH; Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Victoria 3052 Australia nicolas.voelcker@monash.edu tommy.tong@monash.edu.; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Clayton Victoria 3168 Australia.; Melbourne Centre for Nanofabrication, Victorian Node of the Australian National Fabrication Facility Clayton Victoria 3168 Australia.; Department of Materials Science & Engineering, Monash University Clayton Victoria 3168 Australia.
Jazyk:	angličtina
Zdroj:	Nanoscale advances [Nanoscale Adv] 2020 Dec 18; Vol. 3 (3), pp. 682-691. Date of Electronic Publication: 2020 Dec 18 (Print Publication: 2021).
DOI:	10.1039/d0na00857e
Abstrakt:	Nanomaterials are widely used in industrial and clinical settings due to their unique physical and chemical properties. However, public health and environmental concerns have emerged owing to their undesired toxicity and ability to trigger immune responses. This paper presents the development of a microfluidic-based cell biochip device that enables the administration of nanoparticles under laminar flow to cells of the immune system to assess their cytotoxicity. The exposure of human B lymphocytes to 10 nm silver nanoparticles under fluid flow led to a 3-fold increase in toxicity compared to static conditions, possibly indicating enhanced cell-nanoparticle interactions. To investigate whether the administration under flow was the main contributing factor, we compared and validated the cytotoxicity of the same nanoparticles in different platforms, including the conventional well plate format and in-house fabricated microfluidic devices under both static and dynamic flow conditions. Our results suggest that commonly employed static platforms might not be well-suited to perform toxicological screening of nanomaterials and may lead to an underestimation of cytotoxic responses. The simplicity of the developed flow system makes this setup a valuable tool to preliminary screen nanomaterials. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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