Selective photothermal killing of cancer cells using LED-activated nucleus targeting fluorescent carbon dots.

Autor:	Hill SA; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk., Sheikh S; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk., Zhang Q; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk., Sueiro Ballesteros L; School of Cellular and Molecular Medicine, Faculty of Life Sciences Flow Cytometry Facility University Walk Bristol UK., Herman A; School of Cellular and Molecular Medicine, Faculty of Life Sciences Flow Cytometry Facility University Walk Bristol UK., Davis SA; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk., Morgan DJ; Cardiff Catalysis Institute, School of Chemistry, Cardiff University Park Place Cardiff UK., Berry M; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk., Benito-Alifonso D; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk., Galan MC; School of Chemistry, University of Bristol Cantock's Close Bristol UK M.C.Galan@bristol.ac.uk.
Jazyk:	angličtina
Zdroj:	Nanoscale advances [Nanoscale Adv] 2019 Jul 15; Vol. 1 (8), pp. 2840-2846. Date of Electronic Publication: 2019 Jul 15 (Print Publication: 2019).
DOI:	10.1039/c9na00293f
Abstrakt:	The development of effective theranostic probes in cancer therapy is hampered due to issues with selectivity and off-target toxicity. We report the selective LED-photothermal ablation of cervical (HeLa) cancer cells over human dermal fibroblasts (HDF) using a new class of green-emissive fluorescent carbon dots (FCDs). The FCDs can be easily prepared in one pot using cheap and commercial starting materials. Physico-chemical characterization revealed that a surface coating of 2,5-deoxyfructosazine on a robust amorphous core gives rise to the nanomaterial's unique properties. We show that intracellular uptake mostly involves passive mechanisms in combination with intracellular DNA interactions to target the nucleus and that cancer cell selective killing is likely due to an increase in intracellular temperature in combination with ATP depletion, which is not observed upon exposure to either the "naked" core FCDs or the surface components individually. The selectivity of these nanoprobes and the lack of apparent production of toxic metabolic byproducts make these new nanomaterials promising agents in cancer therapy. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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