Tuning protein adsorption on graphene surfaces via laser-induced oxidation.

Autor:	Sitsanidis ED; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi., Schirmer J; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi., Lampinen A; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi., Mentel KK; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi., Hiltunen VM; Department of Physics, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland., Ruokolainen V; Department of Biological and Environmental Sciences, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland., Johansson A; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi.; Department of Physics, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland., Myllyperkiö P; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi., Nissinen M; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi., Pettersson M; Department of Chemistry, Nanoscience Center, University of Jyväskylä P. O. Box 35, FI-40014 JYU Finland mika.j.pettersson@jyu.fi.
Jazyk:	angličtina
Zdroj:	Nanoscale advances [Nanoscale Adv] 2021 Feb 22; Vol. 3 (7), pp. 2065-2074. Date of Electronic Publication: 2021 Feb 22 (Print Publication: 2021).
DOI:	10.1039/d0na01028f
Abstrakt:	An approach for controlled protein immobilization on laser-induced two-photon (2P) oxidation patterned graphene oxide (GO) surfaces is described. Selected proteins, horseradish peroxidase (HRP) and biotinylated bovine serum albumin (b-BSA) were successfully immobilized on oxidized graphene surfaces, via non-covalent interactions, by immersion of graphene-coated microchips in the protein solution. The effects of laser pulse energy, irradiation time, protein concentration and duration of incubation on the topography of immobilized proteins and consequent defects upon the lattice of graphene were systemically studied by atomic force microscopy (AFM) and Raman spectroscopy. AFM and fluorescence microscopy confirmed the selective aggregation of protein molecules towards the irradiated areas. In addition, the attachment of b-BSA was detected by a reaction with fluorescently labelled avidin-fluorescein isothiocyanate (Av-FITC). In contrast to chemically oxidized graphene, laser-induced oxidation introduces the capability for localization on oxidized areas and tunability of the levels of oxidation, resulting in controlled guidance of proteins by light over graphene surfaces and progressing towards graphene microchips suitable for biomedical applications. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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