Polycationic gold nanorods as multipurpose in vitro microtubule markers.

Autor:	Wedler V; Eberhard Karls Universität Tübingen, Cellular Nanoscience (ZMBP) Auf der Morgenstelle 32 72076 Tübingen Germany erik.schaeffer@uni-tuebingen.de +49 7071 295042 +49 7071 2978831., Strauß F; Eberhard Karls Universität Tübingen, Cellular Nanoscience (ZMBP) Auf der Morgenstelle 32 72076 Tübingen Germany erik.schaeffer@uni-tuebingen.de +49 7071 295042 +49 7071 2978831., Sudhakar S; Eberhard Karls Universität Tübingen, Cellular Nanoscience (ZMBP) Auf der Morgenstelle 32 72076 Tübingen Germany erik.schaeffer@uni-tuebingen.de +49 7071 295042 +49 7071 2978831., Hermsdorf GL; Eberhard Karls Universität Tübingen, Cellular Nanoscience (ZMBP) Auf der Morgenstelle 32 72076 Tübingen Germany erik.schaeffer@uni-tuebingen.de +49 7071 295042 +49 7071 2978831., Stierhof YD; Eberhard Karls Universität Tübingen, Cellular Nanoscience (ZMBP) Auf der Morgenstelle 32 72076 Tübingen Germany erik.schaeffer@uni-tuebingen.de +49 7071 295042 +49 7071 2978831., Schäffer E; Eberhard Karls Universität Tübingen, Cellular Nanoscience (ZMBP) Auf der Morgenstelle 32 72076 Tübingen Germany erik.schaeffer@uni-tuebingen.de +49 7071 295042 +49 7071 2978831.
Jazyk:	angličtina
Zdroj:	Nanoscale advances [Nanoscale Adv] 2020 Jul 13; Vol. 2 (9), pp. 4003-4010. Date of Electronic Publication: 2020 Jul 13 (Print Publication: 2020).
DOI:	10.1039/d0na00406e
Abstrakt:	Gold nanoparticles are intriguing because of their unique size- and shape-dependent chemical, electronic and optical properties. Gold nanorods (AuNRs) are particularly promising for various sensor applications due to their tip-enhanced plasmonic fields. For biomolecule attachment, AuNRs are often functionalized with proteins. However, by their intrinsic size such molecules block the most sensitive near-field region of the AuNRs. Here, we used short cationic thiols to functionalize AuNRs. We show that the functionalization layer is thin and that these polycationic AuNRs bind in vitro to negatively charged microtubules. Furthermore, we can plasmonically stimulate light emission from single AuNRs in the absence of any fluorophores and, therefore, use them as bleach- and blinkfree microtubule markers. We expect that polycationic AuNRs may be applicable to in vivo systems and other negatively charged molecules like DNA. In the long-term, microtubule-bound AuNRs can be used as ultrasensitive single-molecule sensors for molecular machines that interact with microtubules. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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