The trajectory patterns of single HIV-1 virus-like particle in live CD4 cells: A real time three-dimensional multi-resolution microscopy study using encapsulated nonblinking giant quantum dot.

Autor:	Li WY; Laboratory of Important Infectious Diseases and Cancer, Department of Medicine, School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan., Yin S; Department of Chemistry, Princeton University, Princeton, NJ 08544, USA., Huang SW; Division of Infectious Diseases, Anschutz Medical Campus, University of Colorado School of Medicine, Aurora, CO, 80045, USA., Yang MH; Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan., Chen PM; College of Medicine, California Northstate University, Elk Grove, CA 95757, USA., Wu SR; Institute of Oral Medicine, National Cheng Kung University, Tainan 701, Taiwan., Welsher K; French Family Science Center, Department of Chemistry, 124 Science Drive, Duke University, Durham, NC 27708, USA., Yang H; Department of Chemistry, Princeton University, Princeton, NJ 08544, USA. Electronic address: hawyang@princeton.edu., Arthur Chen YM; Laboratory of Important Infectious Diseases and Cancer, Department of Medicine, School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan; School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli County 350, Taiwan. Electronic address: 150110@mail.fju.edu.tw.
Jazyk:	angličtina
Zdroj:	Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi [J Microbiol Immunol Infect] 2023 Apr; Vol. 56 (2), pp. 257-266. Date of Electronic Publication: 2022 Aug 28.
DOI:	10.1016/j.jmii.2022.08.011
Abstrakt:	Background: The exploration of virology knowledge was limited by the optical technology for the observation of virus. Previously, a three-dimensional multi-resolution real-time microscope system (3D-MRM) was developed to observe the uptake of HIV-1-tat peptide-modified nanoparticles in cell membrane. In this study, we labeled HIV-1 virus-like particles (VLPs) with passivated giant quantum dots (gQDs) and recorded their interactive trajectories with human Jurkat CD4 cells through 3D-MRM. Methods: The labeled of gQDs of the HIV-1 VLPs in sucrose-gradient purified viral lysates was first confirmed by Cryo-electronic microscopy and Western blot assay. After the infection with CD4 cells, the gQD-labeled VLPs were visualized and their extracellular and intracellular trajectories were recorded by 3D-MRM. Results: A total of 208 prime trajectories was identified and classified into three distinct patterns: cell-free random diffusion pattern, directional movement pattern and cell-associated movement pattern, with distributions and mean durations were 72.6%/87.6 s, 9.1%/402.7 s and 18.3%/68.7 s, respectively. Further analysis of the spatial-temporal relationship between VLP trajectories and CD4 cells revealed the three stages of interactions: (1) cell-associated (extracellular) diffusion stage, (2) cell membrane surfing stage and (3) intracellular directional movement stage. Conclusion: A complete trajectory of HIV-1 VLP interacting with CD4 cells was presented in animation. This encapsulating method could increase the accuracy for the observation of HIV-1-CD4 cell interaction in real time and three dimensions. (Copyright © 2022. Published by Elsevier B.V.)
Databáze:	MEDLINE
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