Fate of intracellular, extracellular polymeric substances-associated, and cell-free antibiotic resistance genes in anaerobic digestion of thermally hydrolyzed sludge.

Autor: Haffiez N; Civil and Environmental Engineering, University of Alberta, 116 Street NW, Edmonton, AB T6G 1H9, Canada., Zakaria BS; Civil and Environmental Engineering, University of Alberta, 116 Street NW, Edmonton, AB T6G 1H9, Canada., Azizi SMM; Civil and Environmental Engineering, University of Alberta, 116 Street NW, Edmonton, AB T6G 1H9, Canada., Dhar BR; Civil and Environmental Engineering, University of Alberta, 116 Street NW, Edmonton, AB T6G 1H9, Canada. Electronic address: bipro@ualberta.ca.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Jan 10; Vol. 855, pp. 158847. Date of Electronic Publication: 2022 Sep 17.
DOI: 10.1016/j.scitotenv.2022.158847
Abstrakt: Thermal hydrolysis of sludge is a promising approach to mitigate antibiotic resistance genes (ARGs) propagation in anaerobic digestion (AD). Although ARGs in sludge may be fractioned into intracellular, extracellular polymeric substance (EPS)-associated, and cell-free ARGs, the fate of these different fractions in AD has never been investigated. This study presents a detailed characterization of intracellular and extracellular ARGs in AD of sludge thermally hydrolyzed at 90 °C and 140 °C. EPS-associated ARGs represented the major fraction of the total extracellular ARGs in all samples, while its lowest abundance was observed for thermal hydrolysis at 140 °C along with the lowest EPS levels. The results suggested a positive correlation between EPS-associated ARGs with intracellular and cell-free ARGs. Furthermore, various EPS components, such as proteins and e-DNA, were positively correlated with β-lactam resistance genes. sul1 dominated all samples as an EPS-associated resistance gene. These results provide new insights into the significance of different ARGs fractions in their overall dissemination in AD integrated with thermal hydrolysis.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interests or personal relationships that could influence this research work.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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