FOXO1 is downregulated in obese mice subjected to short-term strength training.

Autor: Pereira RM; School of Applied Sciences, Exercise Cell Biology Lab, School of Applied Sciences, University of Campinas, Limeira, Brazil.; School of Applied Sciences, Laboratory of Molecular Biology of Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., da Cruz Rodrigues KC; School of Applied Sciences, Exercise Cell Biology Lab, School of Applied Sciences, University of Campinas, Limeira, Brazil.; School of Applied Sciences, Laboratory of Molecular Biology of Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., Sant'Ana MR; Nutrition Division, Laboratory of Nutritional Genomics, School of Applied Sciences, University of Campinas, Limeira, Brazil., da Rocha AL; Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School and Postgraduate Program in Physical Education and Sport, School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Morelli AP; Health Division, Multidisciplinary Laboratory of Food and Health, School of Applied Sciences, University of Campinas, Limeira, Brazil., Veras ASC; Department of Physical Education, School of Technology and Sciences, Postgraduate Program in Multicentric Physiological Sciences, São Paulo State University-UNESP, campus of Aracatuba, Presidente Prudente, Brazil.; Experimental Laboratory of Exercise Biology, State University of São Paulo-UNESP, Presidente Prudente, Brazil., Gaspar RS; Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, State University of Campinas, Campinas, Brazil., da Costa Fernandes CJ; School of Applied Sciences, Exercise Cell Biology Lab, School of Applied Sciences, University of Campinas, Limeira, Brazil., Teixeira GR; Department of Physical Education, School of Technology and Sciences, Postgraduate Program in Multicentric Physiological Sciences, São Paulo State University-UNESP, campus of Aracatuba, Presidente Prudente, Brazil.; Experimental Laboratory of Exercise Biology, State University of São Paulo-UNESP, Presidente Prudente, Brazil., Simabuco FM; Health Division, Multidisciplinary Laboratory of Food and Health, School of Applied Sciences, University of Campinas, Limeira, Brazil.; Department of Biochemistry, Federal University of São Paulo, São Paulo, Brazil., da Silva ASR; Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School and Postgraduate Program in Physical Education and Sport, School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Cintra DE; Nutrition Division, Laboratory of Nutritional Genomics, School of Applied Sciences, University of Campinas, Limeira, Brazil., Ropelle ER; School of Applied Sciences, Exercise Cell Biology Lab, School of Applied Sciences, University of Campinas, Limeira, Brazil.; School of Applied Sciences, Laboratory of Molecular Biology of Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., Pauli JR; School of Applied Sciences, Exercise Cell Biology Lab, School of Applied Sciences, University of Campinas, Limeira, Brazil.; School of Applied Sciences, Laboratory of Molecular Biology of Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., de Moura LP; School of Applied Sciences, Exercise Cell Biology Lab, School of Applied Sciences, University of Campinas, Limeira, Brazil.; School of Applied Sciences, Laboratory of Molecular Biology of Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2022 Nov; Vol. 237 (11), pp. 4262-4274. Date of Electronic Publication: 2022 Sep 20.
DOI: 10.1002/jcp.30882
Abstrakt: Obesity is a worldwide health problem and is directly associated with insulin resistance and type 2 diabetes. The liver is an important organ for the control of healthy glycemic levels, since insulin resistance in this organ reduces phosphorylation of forkhead box protein 1 (FOXO1) protein, leading to higher hepatic glucose production (HGP) and fasting hyperglycemia. Aerobic physical training is known as an important strategy in increasing the insulin action in the liver by increasing FOXO1 phosphorylation and reducing gluconeogenesis. However, little is known about the effects of strength training in this context. This study aimed to investigate the effects of short-term strength training on hepatic insulin sensitivity and glycogen synthase kinase-3β (GSK3β) and FOXO1 phosphorylation in obese (OB) mice. To achieve this goal, OB Swiss mice performed the strength training protocol (one daily session for 15 days). Short-term strength training increased the phosphorylation of protein kinase B and GSK3β in the liver after insulin stimulus and improved the control of HGP during the pyruvate tolerance test. On the other hand, sedentary OB animals reduced FOXO1 phosphorylation and increased the levels of nuclear FOXO1 in the liver, increasing the phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) content. The bioinformatics analysis also showed positive correlations between hepatic FOXO1 levels and gluconeogenic genes, reinforcing our findings. However, strength-trained animals reverted to this scenario, regardless of body adiposity changes. In conclusion, short-term strength training is an efficient strategy to enhance the insulin action in the liver of OB mice, contributing to glycemic control by reducing the activity of hepatic FOXO1 and lowering PEPCK and G6Pase contents.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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