The molecular characteristic analysis of TRIB2 gene and its expressional patterns in Bos grunniens tissue and granulosa cells.

Autor: Zhao D; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Wu J; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China., Ma Y; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Zhang J; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Feng X; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Fan Y; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Xiong X; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Fu W; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Li J; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China., Xiong Y; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, Sichuan Province, China.; Key Laboratory for Animal Science of National Ethnic Affairs Commission, Chengdu, Sichuan, China.
Jazyk: angličtina
Zdroj: Animal biotechnology [Anim Biotechnol] 2023 Dec; Vol. 34 (7), pp. 2846-2854. Date of Electronic Publication: 2022 Sep 20.
DOI: 10.1080/10495398.2022.2121716
Abstrakt: Tribbles homolog 2 (TRIB2) plays an important role in the follicular development of female mammals. However, its expression and function in the yak ( Bos grunniens ) are still unclear. In this study, we predicted the molecular characteristics of TRIB2 , and revealed its expression pattern in yak ( Bos grunniens ) tissues and ovarian granulosa cells. We cloned the full length of the yak TRIB2 gene obtained by RT-PCR was 1368 bp and the coding sequence (CDS) was 624 bp, encoding 207 amino acids (AA). Homology analysis showed that the yak TRIB2 is highly conserved among species. TRIB2 was detected to be extensively expressed in seven tissues of the yak liver, spleen, lung, kidney, ovary, oviduct and uterus by qPCR. The expression of TRIB2 mRNA in the ovary during gestation was significantly lower than that in the non-pregnant ( p  < 0.05). At each stage of follicle development, the TRIB2 mRNA in granulosa cells showed a significant upward trend with the development of follicles. The expression of TRIB2 gradually decreased with the increase of the culture time of the granulosa cells in vitro . In conclusion, these results suggest that TRIB2 may play an important role in the follicular development of yaks.
Databáze: MEDLINE
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