Effects of different protocols of defocused high-power laser on the viability and migration of myoblasts-a comparative in vitro study.

Autor: Brochado FT; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil., Mármora BC; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil., Campos PS; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil., Schmidt TR; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil., Fernandes KPS; Postgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University - UNINOVE, São Paulo, SP, Brazil., Bussadori SK; Postgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University - UNINOVE, São Paulo, SP, Brazil., Santos LG; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil., Wagner VP; Academic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK.; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil., Lamers ML; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil., Martins MD; Department of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil. manomartins@gmail.com.; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil. manomartins@gmail.com.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2022 Dec; Vol. 37 (9), pp. 3571-3581. Date of Electronic Publication: 2022 Sep 20.
DOI: 10.1007/s10103-022-03636-7
Abstrakt: The aim of the present study was to analyze for the first time the effect of photobiomodulation therapy (PBMT) using defocused high-power laser (DHPL) in myoblast cell line C2C12 viability and migration and compare them with low-power laser therapy. Cells were divided into 9 groups: Sham irradiation 10% fetal bovine serum (FBS); Sham irradiation 5%FBS; low-power laser 0.1 W; DHPL 810 1 W; DHPL 810 2 W; DHPL 980 1 W; DHPL 980 2 W; DHPL dual 1 W; DHPL dual 2 W. To simulate stress conditions, all groups exposed to irradiation were maintained in DMEM 5% FBS. The impact of therapies on cell viability was assessed through sulforhodamine B assay and on cells migration through scratch assays and time-lapse. Myoblast viability was not modified by PBMT protocols. All PBMT protocols were able to accelerate the scratch closure after 6 and 18 h of the first irradiation (p < 0.001). Also, an increase in migration speed, with a more pronounced effect of DHPL laser using dual-wavelength protocol with 2 W was observed (p < 0.001). In conclusion, the diverse PBMT protocols used in this study accelerated the C2C12 myoblasts migration, with 2-W dual-wavelength outstanding as the most effective protocol tested. Benefits from treating muscle injuries with PBMT appear to be related to its capacity to induce cell migration without notable impact on cell viability.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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