A novel membraneless β-glucan/O 2 enzymatic fuel cell based on β-glucosidase (RmBgl3B)/pyranose dehydrogenase (AmPDH) co-immobilized onto buckypaper electrode.

Autor: Rafighi P; College of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box 45195-1159, Gava Zang, Zanjan, Iran., Nordberg Karlsson E; Biotechnology, Department of Chemistry, Lund University, Lund S-22100, Sweden., Zubaida Gulshan Ara K; Biotechnology, Department of Chemistry, Lund University, Lund S-22100, Sweden., Pankratova G; National Centre for Nano Fabrication and Characterization, Technical University of Denmark (DTU), Kongens Lyngby, Denmark., Bollella P; Department of Chemistry, University of Bari A. Moro, Via E. Orabona 4, 70125 Bari, Italy., Peterbauer CK; Department of Food Sciences and Technology BOKU-University of Natural Resources and Life Sciences Muthgasse 18, A-1190 Wien, Austria., Gorton L; Department of Biochemistry and Structural Biology, Lund University, PO Box 124, 221 00 Lund, Sweden. Electronic address: lo.gorton@biochemistry.lu.se.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2022 Dec; Vol. 148, pp. 108254. Date of Electronic Publication: 2022 Sep 05.
DOI: 10.1016/j.bioelechem.2022.108254
Abstrakt: A novel membraneless β-glucan/O 2 enzymatic fuel cell was developed by combining a bioanode based on buckypaper modified with co-immobilized Agaricus meleagris pyranose dehydrogenase (AmPDH) and Rhodothermus marinus β-glucosidase (RmBgl3B) (RmBgl3B-AmPDH/buckypaper) with a biocathode based on solid graphite modified with Myrothecium verrucaria bilirubin oxidase (MvBOx/graphite). AmPDH was connected electrochemically with the buckypaper using an osmium redox polymer in a mediated reaction, whereas MvBOx was connected with graphite in a direct electron transfer reaction. The fuel for the bioanode was produced by enzymatic hydrolysis of β-glucan by the exoglucanase RmBgl3B into d-glucose, which in turn was enzymatically oxidised by AmPDH to generate a current response. This design allows to obtain an efficient enzymatic fuel cell, where the chemical energy converted into electrical energy is higher than the chemical energy stored in complex carbohydrate based fuel. The maximum power density of the assembled β-glucan/O 2 biofuel cell reached 26.3 ± 4.6 μWcm -2 at 0.36 V in phosphate buffer containing 0.5 % (w/v) β-glucan at 40 °C with excellent stability retaining 68.6 % of its initial performance after 5 days. The result confirms that β-glucan can be employed as fuel in an enzymatic biofuel cell.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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