Discovery and identification of quality markers of Sparganii Rhizoma based on zebrafish thrombosis model.

Autor: Xu N; Laboratory of Chinese Medicine Preparation, Shandong Research Academy of Traditional Chinese Medicine, Jinan 250014, China., Sun R; Institute of Advanced Medical Sciences, Shandong University, Jinan 250014, China., Shi YP; Department of Clinical Pharmacy, The First Affiliated Hospital of Shandong First Medical University, Jinan 250014, China., Han LW; Institute of Biology of Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China., Shi HY; Department of Clinical Pharmacy, The First Affiliated Hospital of Shandong First Medical University, Jinan 250014, China.
Jazyk: angličtina
Zdroj: Chinese herbal medicines [Chin Herb Med] 2021 Apr 27; Vol. 13 (3), pp. 389-395. Date of Electronic Publication: 2021 Apr 27 (Print Publication: 2021).
DOI: 10.1016/j.chmed.2021.04.015
Abstrakt: Objective: The aim of the present study was to determine the quality marker (Q-Markers) of Sparganii Rhizoma against thrombus through an integration of investigations on its antithrombotic effect, content determination and spectrum-effect correlation analysis.
Methods: Based on the concept of Q-Marker, Sparganii Rhizoma was investigated for the identification of chemical component. The pharmacological effects on arachidonic acid-induced thrombosis in zebrafish were also investigated. The material basis in ethanol extract was determined by HPLC-UV. Furthermore, the potential Q-Markers were analyzed and predicted according to the effect-chemical correlation analysis. Finally, the anti-thrombotic Q-Markers were verified through the anti-thrombotic test of monomer components.
Results: The model of thrombosis zebrafish was established with larvae exposed to 100 µmol/L arachidonic acid for 1 h. Nine ingredients in Sparganii Rhizoma were identified as 5-hydroxymethylfurfural, vanillic acid, ferulic acid, p -hydroxybenzaldehyde, p -hydroxybenzoic acid, vanillin, protocatechuic acid, p -coumaric acid and isoferulic acid. According to the determination effect of zebrafish thrombosis model and HPLC content analysis results, all the other contents present positive correlation except 5-hydroxymethylfurfural, and the P values of three representative potential Q-Markers (ferulic acid, protocatechuic acid and p -coumaric acid) were 0.002, 0.001 and 0.026, respectively.
Conclusion: Sparganii Rhizoma showed a dose-dependent effect on the recovery of reducing cardiac red blood cell on zebrafish model. Three phenolic acids (ferulic acid, protocatechuic acid and p -coumaric acid) were proved to possess the anti-thrombotic effects which could be regarded as the potential Q-Markers for quality assessment of Sparganii Rhizoma .
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2021 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V.)
Databáze: MEDLINE
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