Co-cultured adventitious roots of Echinacea pallida and Echinacea purpurea inhibit lipopolysaccharide-induced inflammation via MAPK pathway in mouse peritoneal macrophages.

Autor: Fan MZ; College of Agriculture, Yanbian University, Yanji 133002, China., Wu XH; College of Agriculture, Yanbian University, Yanji 133002, China., Li XF; College of Agriculture, Yanbian University, Yanji 133002, China., Piao XC; College of Agriculture, Yanbian University, Yanji 133002, China., Jiang J; College of Agriculture, Yanbian University, Yanji 133002, China., Lian ML; College of Agriculture, Yanbian University, Yanji 133002, China.
Jazyk: angličtina
Zdroj: Chinese herbal medicines [Chin Herb Med] 2021 Jan 26; Vol. 13 (2), pp. 228-234. Date of Electronic Publication: 2021 Jan 26 (Print Publication: 2021).
DOI: 10.1016/j.chmed.2021.01.001
Abstrakt: Objective: In order to elucidate the biological activity of the co-cultured adventitious roots (ARs) of Echinacea pallida and Echinacea purpurea and provide theoretical basis for its application, and the anti-inflammatory activities and potential mechanisms of co-cultured ARs were studied.
Methods: The experimental materials were obtained by bioreactor co-culture technology and used in the activity research. In this study, mouse macrophages induced by lipopolysaccharide (LPS) were used as in vitro model. Different concentrations of AR extract (50-400 g/mL) were used to treat cells. The expression of pro-inflammatory cytokines was determined using enzyme linked immunosorbent assay. The inducible nitric oxide synthase and cyclooxygenase-2 expression, mitogen-activated protein kinase (MAPK) phosphorylation, and the inhibitor of nuclear factor-kappa B-α levels were determined by the Western blot analysis.
Results: In the co-cultured ARs, total flavonoids and total caffeic acid were determined, and the contents of both bioactive compounds were significantly higher than those ARs from the single-species culture. Compared with the control group, the large amount of pro-inflammatory mediators was released after LPS stimulation. However, in the extract groups with different concentrations (25, 50, and 100 g/mL), the production of these pro-inflammatory mediators was inhibited in a dose-dependent manner. Furthermore, the levels of phosphorylation of MAPK proteins, including p-p38, p-c-Jun N-terminal kinase, and p-extracellular regulated protein kinases were significantly ( P  < 0.05) decreased in the extract groups, revealing that the AR extract probably involved in regulating the MAPK signaling pathway.
Conclusion: Collectively, our findings suggested that the co-cultured ARs of E. pallida and E. purpurea can inhibit production of pro-inflammatory mediators in mouse peritoneal macrophages and possess the anti-inflammatory effect by regulating MAPK signaling pathways.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2021 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V.)
Databáze: MEDLINE
Externí odkaz: