Saprophytic bacteria and fungi colonize stearoyl coenzyme-A desaturase-1 knockout skin.

Autor: Pyle HJ; Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Artami M; Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Edwards M; Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Raj P; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Zhang B; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Arana C; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Harris-Tryon TA; Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Jazyk: angličtina
Zdroj: Experimental dermatology [Exp Dermatol] 2023 Jan; Vol. 32 (1), pp. 78-84. Date of Electronic Publication: 2022 Oct 02.
DOI: 10.1111/exd.14676
Abstrakt: Lipids synthesized on the skin are critical to the antimicrobial barrier. Skin lipids also facilitate survival of lipophilic skin commensals in an otherwise dry and acidic ecological landscape. Thus, skin-specific stearoyl-coenzyme A desaturase 1 knockout mice (Scd1 ΔK14 ) with sebocyte atrophy and decreased synthesis of monounsaturated fatty acids, triglycerides and wax diesters have dry, inflamed skin. Here, we used 16S rRNA (V1-V2 and V1-V9) and internal transcribed spacer 1 (ITS1) amplicon sequencing to compare bacterial and fungal skin microbiomes between Scd1 ΔK14 mice and wildtype control mice (Scd1 fl/fl ) in a barrier facility. Saprophytic bacteria including Sporosarcina spp. and Staphylococcus lentus and saprophytic fungi including Alternaria infectoria were found in higher relative abundance in the Scd1 ΔK14 group (ANCOM). Analysis of community diversity (Shannon index) revealed greater fungal alpha diversity in the Scd1 ΔK14 group (p = 0.009, Kruskal-Wallis). Principal coordinates analysis (Bray-Curtis dissimilarity) showed that both bacterial (p = 0.002, PERMANOVA) and fungal communities (p = 0.006, PERMANOVA) of the Scd1 ΔK14 group were unique from the wildtype group. Altogether, these results suggest that sebaceous gland-derived lipids normally restrict the skin microbiome, and in the absence of these lipids, a greater diversity of opportunistic organisms are able to colonize the surface of skin.
(© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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