Reconstituted TAD-size chromatin fibers feature heterogeneous nucleosome clusters.

Autor: Korolev N; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore., Zinchenko A; Graduate School of Environmental Studies, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, 464-8601, Japan., Soman A; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore., Chen Q; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore., Wong SY; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.; Department of Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 169857, Singapore., Berezhnoy NV; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore., Basak R; Department of Physics, National University of Singapore, Singapore, 117542, Singapore., van der Maarel JRC; Department of Physics, National University of Singapore, Singapore, 117542, Singapore., van Noort J; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore. noort@physics.leidenuniv.nl.; Huygens-Kamerlingh Ohnes Laboratory, Leiden University, Niels Bohrweg 2, 2333 CA, Leiden, The Netherlands. noort@physics.leidenuniv.nl., Nordenskiöld L; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore. larsnor@ntu.edu.sg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Sep 16; Vol. 12 (1), pp. 15558. Date of Electronic Publication: 2022 Sep 16.
DOI: 10.1038/s41598-022-19471-3
Abstrakt: Large topologically associated domains (TADs) contain irregularly spaced nucleosome clutches, and interactions between such clutches are thought to aid the compaction of these domains. Here, we reconstituted TAD-sized chromatin fibers containing hundreds of nucleosomes on native source human and lambda-phage DNA and compared their mechanical properties at the single-molecule level with shorter '601' arrays with various nucleosome repeat lengths. Fluorescent imaging showed increased compaction upon saturation of the DNA with histones and increasing magnesium concentration. Nucleosome clusters and their structural fluctuations were visualized in confined nanochannels. Force spectroscopy revealed not only similar mechanical properties of the TAD-sized fibers as shorter fibers but also large rupture events, consistent with breaking the interactions between distant clutches of nucleosomes. Though the arrays of native human DNA, lambda-phage and '601' DNA featured minor differences in reconstitution yield and nucleosome stability, the fibers' global structural and mechanical properties were similar, including the interactions between nucleosome clutches. These single-molecule experiments quantify the mechanical forces that stabilize large TAD-sized chromatin domains consisting of disordered, dynamically interacting nucleosome clutches and their effect on the condensation of large chromatin domains.
(© 2022. The Author(s).)
Databáze: MEDLINE
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