| Autor: |
Contino M; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Leonardi C; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Genovese C; National Research Council, Institute for Agricultural and Forest Systems in the Mediterranean, Catania, Italy., Scalisi EM; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Pecoraro R; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Ignoto S; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Failla C; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Ferruggia G; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy., Salvaggio A; Experimental Zooprophylactic Institute of Sicily 'A. Mirri', Catania, Italy., Asero P; Centro Biomedico ASTER, Catania, Italy., Calderaro P; National Research Council, Institute for Agricultural and Forest Systems in the Mediterranean, Catania, Italy., Toscano V; National Research Council, Institute for Agricultural and Forest Systems in the Mediterranean, Catania, Italy., Raccuia SA; National Research Council, Institute for Agricultural and Forest Systems in the Mediterranean, Catania, Italy., Caruso G; U.O. Fisiopatologia della Riproduzione Umana - Clinica del Mediterraneo, Ragusa, Italy., Bracchitta G; U.O. Fisiopatologia della Riproduzione Umana - Clinica del Mediterraneo, Ragusa, Italy., Brundo MV; Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy. |
| Abstrakt: |
This study investigated the phenolic compounds and antioxidant capacity of fruit extracts of Opuntia dillenii (Ker Gawl.) Haw.(OD) and Opuntia ficus-indica (L.) Mill.(OFI), yellow (F1) and red (F2) varieties. In order to evaluate the antioxidant activity of these extracts on human sperm quality after thawing, the semen parameters (vitality, motility, acrosome reaction, oxidative stress, and DNA fragmentation) were analysed after 1 h of exposure. The results showed that OD has higher phenolic content and antioxidant power than OFI, and that they are higher in F2 than F1. Furthermore, regarding the activity of extracts on thawed sperm, the results showed a significant increase in motility in samples treated with OFI F1 and OD extracts, while an improvement in vitality and acrosome reaction and a reduction of DNA fragmentation were observed in all exposed samples compared to the control. Finally, a reduction of oxidative stress was observed in samples exposed to OFI F2 and OD than control. |
