Genome assembly and chemogenomic profiling of National Flower of Singapore Papilionanthe Miss Joaquim 'Agnes' reveals metabolic pathways regulating floral traits.

Autor: Lim AH; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore., Low ZJ; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore., Shingate PN; Genome Institute of Singapore, A*STAR, Singapore, Singapore., Hong JH; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Chong SC; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore., Ng CCY; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore., Liu W; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore., Vaser R; Genome Institute of Singapore, A*STAR, Singapore, Singapore.; Faculty of Electrical Engineering and Computing, University of Zagreb, Zagreb, Croatia., Šikić M; Genome Institute of Singapore, A*STAR, Singapore, Singapore.; Faculty of Electrical Engineering and Computing, University of Zagreb, Zagreb, Croatia., Sung WK; Genome Institute of Singapore, A*STAR, Singapore, Singapore.; School of Computing, National University of Singapore, Singapore, Singapore., Nagarajan N; Genome Institute of Singapore, A*STAR, Singapore, Singapore.; School of Computing, National University of Singapore, Singapore, Singapore., Tan P; Genome Institute of Singapore, A*STAR, Singapore, Singapore. tanbop@gis.a-star.edu.sg.; Duke-NUS Medical School, Singapore, Singapore. tanbop@gis.a-star.edu.sg.; Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore. tanbop@gis.a-star.edu.sg.; SingHealth/Duke-NUS Institute of Precision Medicine, Singapore, Singapore. tanbop@gis.a-star.edu.sg., Teh BT; SingHealth Duke-NUS Institute of Biodiversity Medicine, Singapore, Singapore. teh.bin.tean@singhealth.com.sg.; Genome Institute of Singapore, A*STAR, Singapore, Singapore. teh.bin.tean@singhealth.com.sg.; Duke-NUS Medical School, Singapore, Singapore. teh.bin.tean@singhealth.com.sg.; SingHealth/Duke-NUS Institute of Precision Medicine, Singapore, Singapore. teh.bin.tean@singhealth.com.sg.; Institute of Molecular and Cell Biology, Singapore, Singapore. teh.bin.tean@singhealth.com.sg.; National Cancer Center Singapore, Singapore, Singapore. teh.bin.tean@singhealth.com.sg.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2022 Sep 15; Vol. 5 (1), pp. 967. Date of Electronic Publication: 2022 Sep 15.
DOI: 10.1038/s42003-022-03940-6
Abstrakt: Singapore's National Flower, Papilionanthe (Ple.) Miss Joaquim 'Agnes' (PMJ) is highly prized as a horticultural flower from the Orchidaceae family. A combination of short-read sequencing, single-molecule long-read sequencing and chromatin contact mapping was used to assemble the PMJ genome, spanning 2.5 Gb and 19 pseudo-chromosomal scaffolds. Genomic resources and chemical profiling provided insights towards identifying, understanding and elucidating various classes of secondary metabolite compounds synthesized by the flower. For example, presence of the anthocyanin pigments detected by chemical profiling coincides with the expression of ANTHOCYANIN SYNTHASE (ANS), an enzyme responsible for the synthesis of the former. Similarly, the presence of vandaterosides (a unique class of glycosylated organic acids with the potential to slow skin aging) discovered using chemical profiling revealed the involvement of glycosyltransferase family enzymes candidates in vandateroside biosynthesis. Interestingly, despite the unnoticeable scent of the flower, genes involved in the biosynthesis of volatile compounds and chemical profiling revealed the combination of oxygenated hydrocarbons, including traces of linalool, beta-ionone and vanillin, forming the scent profile of PMJ. In summary, by combining genomics and biochemistry, the findings expands the known biodiversity repertoire of the Orchidaceae family and insights into the genome and secondary metabolite processes of PMJ.
(© 2022. The Author(s).)
Databáze: MEDLINE
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