Massive crossover suppression by CRISPR-Cas-mediated plant chromosome engineering.

Autor: Rönspies M; Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany., Schmidt C; Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany.; Division of Cancer Research, Department of Thoracic Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany., Schindele P; Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany., Lieberman-Lazarovich M; Institute of Plant Sciences, Department of Vegetable and Field Crops, Agricultural Research Organization (ARO), Volcani Center, Rishon LeZion, Israel., Houben A; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany., Puchta H; Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany. holger.puchta@kit.edu.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2022 Oct; Vol. 8 (10), pp. 1153-1159. Date of Electronic Publication: 2022 Sep 15.
DOI: 10.1038/s41477-022-01238-3
Abstrakt: Recent studies have demonstrated that not only genes but also entire chromosomes can be engineered using clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPER-associated protein 9 (Cas9) 1-5 . A major objective of applying chromosome restructuring in plant breeding is the manipulation of genetic exchange 6 . Here we show that meiotic recombination can be suppressed in nearly the entire chromosome using chromosome restructuring. We were able to induce a heritable inversion of a >17 Mb-long chromosome fragment that contained the centromere and covered most of chromosome 2 of the Arabidopsis ecotype Col-0. Only the 2 and 0.5 Mb-long telomeric ends remained in their original orientation. In single-nucleotide polymorphism marker analysis of the offspring of crosses with the ecotype Ler-1, we detected a massive reduction of crossovers within the inverted chromosome region, coupled with a shift of crossovers to the telomeric ends. The few genetic exchanges detected within the inversion all originated from double crossovers. This not only indicates that heritable genetic exchange can occur by interstitial chromosome pairing, but also that it is restricted to the production of viable progeny.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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