Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection.

Autor: Nesa J; Chemical Biology Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, 733134, India., Jana SK; Department of Biochemisty, Bose Institute, P1/12 CIT Scheme, VIIM, Kolkata, 700054, India., Sadat A; Insect Ecology and Conservation Biology Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, 733134, India., Biswas K; Laboratory of Organic Synthesis, Department of Chemistry, Raiganj University, North Dinajpur, 733134, India., Kati A; Experimental Medicine Research and Application Center, University of Health Sciences, Validebag Research Park, Uskudar, 34662, Istanbul, Turkey., Kaya O; TERRA Analysis and Measurement Device Tic. Inc., Istanbul, Turkey., Mondal R; Chemical Biology Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, 733134, India., Dam P; Chemical Biology Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, 733134, India., Thakur M; Laboratory of Organic Synthesis, Department of Chemistry, Raiganj University, North Dinajpur, 733134, India., Kumar A; ANMOL Laboratory, Department of Biotechnology, North Bengal University, Darjeeling, 734013, India., Hossain M; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, India., Lima LR; S-Inova Biotech, Programa de Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, 79117900, Brazil., Rezende SB; S-Inova Biotech, Programa de Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, 79117900, Brazil., Bhattacharjya D; Silkworm Rearing Technology and Extension Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, West Bengal, 733134, India., Gangopadhyay D; Silkworm Genetics and Breeding Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, 733134, India., Ghorai S; Department of Microbiology, Raiganj University, North Dinajpur, 733134, India., Altuntas S; Experimental Medicine Research and Application Center, University of Health Sciences, Validebag Research Park, Uskudar, 34662, Istanbul, Turkey., Panda AK; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, India., Chakrabarti P; Department of Biochemisty, Bose Institute, P1/12 CIT Scheme, VIIM, Kolkata, 700054, India., Swarnakar S; Department of Botany, Raiganj University, North Dinajpur, 733134, India., Chakraborty J; Department of Microbiology, Raiganj University, North Dinajpur, 733134, India., Yilmaz B; Experimental Medicine Research and Application Center, University of Health Sciences, Validebag Research Park, Uskudar, 34662, Istanbul, Turkey., Macedo MLR; Laboratório de Purificação de Proteínas e suas Funções Biológicas, Universidade Federal de Mato Grosso do Sul (UFMS), Cidade Universitária, Campo Grande, Mato Grosso Do Sul, 79070900, Brazil., Franco OL; S-Inova Biotech, Programa de Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, 79117900, Brazil.; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-graduação em Ciências Genômicas e Biotecnologia, UniversidadeCatólica de Brasília (UCB), Brasília, 70790160, Brazil., Cardoso MH; S-Inova Biotech, Programa de Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, 79117900, Brazil. marlonhenrique6@gmail.com.; Laboratório de Purificação de Proteínas e suas Funções Biológicas, Universidade Federal de Mato Grosso do Sul (UFMS), Cidade Universitária, Campo Grande, Mato Grosso Do Sul, 79070900, Brazil. marlonhenrique6@gmail.com.; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-graduação em Ciências Genômicas e Biotecnologia, UniversidadeCatólica de Brasília (UCB), Brasília, 70790160, Brazil. marlonhenrique6@gmail.com., Mandal AK; Chemical Biology Laboratory, Department of Sericulture, Raiganj University, North Dinajpur, 733134, India. amitmandal08@gmail.com.; Centre for Nanotehnology Sciences (CeNS), Raiganj University, North Dinajpur, 733134, India. amitmandal08@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Sep 15; Vol. 12 (1), pp. 15493. Date of Electronic Publication: 2022 Sep 15.
DOI: 10.1038/s41598-022-19450-8
Abstrakt: The main effectors in the innate immune system of Bombyx mori L. are antimicrobial peptides (AMPs). Here, we infected B. mori with varied inoculum sizes of Pseudomonas aeruginosa ATCC 25668 cells to investigate changes in morpho-anatomical responses, physiological processes and AMP production. Ultraviolet-visible spectra revealed a sharp change in λ max from 278 to 285 nm (bathochromic shift) in the hemolymph of infected B. mori incubated for 24 h. Further, Fourier Transform InfraRed studies on the hemolymph extracted from the infected B. mori showed a peak at 1550 cm -1 , indicating the presence of α-helical peptides. The peptide fraction was obtained through methanol, acetic acid and water mixture (90:1:9) extraction, followed by peptide purification using Reverse Phase High Performance Liquid Chromatography. The fraction exhibiting antibacterial properties was collected and characterized by Matrix-Assisted Laser Desorption/Ionization-Time of Flight. A linear α-helical peptide with flexible termini (LLKELWTKMKGAGKAVLGKIKGLL) was found, corresponding to a previously described peptide from ant venom and here denominated as Bm-ponericin-L1. The antibacterial activity of Bm-ponericin-L1 was determined against ESKAPE pathogens. Scanning electron microscopy confirmed the membrane disruption potential of Bm-ponericin-L1. Moreover, this peptide also showed promising antibiofilm activity. Finally, cell viability and hemolytic assays revealed that Bm-ponericin-L1 is non-toxic toward primary fibroblasts cell lines and red blood cells, respectively. This study opens up new perspectives toward an alternative approach to overcoming multiple-antibiotic-resistance by means of AMPs through invertebrates' infection with human pathogenic bacteria.
(© 2022. The Author(s).)
Databáze: MEDLINE
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