First report of Giardia duodenalis assemblage F in humans and dogs in southern Brazil.

Autor: Silva ACDS; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Martins FDC; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Ladeia WA; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Kakimori MTA; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Lucas JI; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Sasse JP; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Martins TA; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil., Macedo VFR; Clinical Parasitology Laboratory of the University Hospital, Ambulatory Hospital of Clínicas, State University of Londrina -UEL, Londrina, PR, Brazil., Murad VA; Clinical Parasitology Laboratory of the University Hospital, Ambulatory Hospital of Clínicas, State University of Londrina -UEL, Londrina, PR, Brazil., de Barros LD; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil; Department of Clinical Veterinary, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, R. Dr. Valter Maurício Corrêa, s/n, 18618-681 Botucatu, SP, Brazil. Electronic address: ldbarros@uel.br., Garcia JL; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, 86057-970 Londrina, PR, Brazil.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2022 Oct; Vol. 89, pp. 101878. Date of Electronic Publication: 2022 Sep 08.
DOI: 10.1016/j.cimid.2022.101878
Abstrakt: The present study aimed to molecularly characterize Giardia duodenalis from stool samples of humans, dogs, and cats. Molecular analyses were performed on 59 samples that tested positive for G. duodenalis on coproparasitological examinations. After extraction, the samples were first tested by nested polymerase chain reaction (n-PCR) analysis of the SSU-rRNA gene, and for the samples that were positive, the β-giardin, TPI, and GDH genes were analyzed. The amplicons obtained in the n-PCR of the β-giardin gene were subjected to PCR-restriction length polymorphism (RFLP) analysis and subsequent digestion with the enzyme HaeIII to differentiate the assemblages. Seven (11.8 %), 34 (57.7 %), and 18 (30.5 %) out of 59 samples were from humans, dogs, and cats, respectively. Nested-PCR results showed that 49.2 % (29/59) of samples were positive for the SSU-rRNA gene, with 42.9 % (3/7) of humans, 55.9 % (19/34) of dogs, and 38.9 % (7/18) of catsve. Of the other genes analyzed, β-giardin was amplified most frequently, in 34.5 % (10/29) of samples, followed by GDH in 27.6 % (8/29) of samples, and TPI in 10.3 % (3/29) of samples. Only one sample from a dog showed the amplification of all genes. PCR-RFLP analysis showed assemblage F in a human, dog, and cat samples; and assemblage C and D in dog samples. This is the first description of assemblage F in humans from Brazil and the first description of assemblage F in dogs. Further studies are needed to verify the frequency with which these infections occur, and provide information that will contribute to the molecular epidemiological understanding of giardiasis.
Competing Interests: Conflict of interest None.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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