Organoselenium compounds as functionalizing agents for gold nanoparticles in cancer therapy.

Autor: Lorenzoni S; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy., Cerra S; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy. Electronic address: sara.cerra@uniroma1.it., Angulo-Elizari E; Department of Pharmaceutical Technology and Chemistry, University of Navarra, Irunlarrea 1, Pamplona E-31008, Spain., Salamone TA; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy., Battocchio C; Department of Sciences, Roma Tre University, Via della Vasca Navale 79, 00146 Rome, Italy., Marsotto M; Department of Sciences, Roma Tre University, Via della Vasca Navale 79, 00146 Rome, Italy., Scaramuzzo FA; Department of Basic and Applied Sciences for Engineering (SBAI), Sapienza University of Rome, Via Antonio Scarpa 14, 00161 Rome, Italy., Sanmartín C; Department of Pharmaceutical Technology and Chemistry, University of Navarra, Irunlarrea 1, Pamplona E-31008, Spain., Plano D; Department of Pharmaceutical Technology and Chemistry, University of Navarra, Irunlarrea 1, Pamplona E-31008, Spain. Electronic address: dplano@unav.es., Fratoddi I; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2022 Nov; Vol. 219, pp. 112828. Date of Electronic Publication: 2022 Sep 07.
DOI: 10.1016/j.colsurfb.2022.112828
Abstrakt: Gold nanoparticles (AuNPs) modified with four organoselenium compounds, i.e., 4-selenocyanatoaniline (compound 1), 4,4'-diselanediyldianiline (compound 2), N-(4-selenocyanatophenyl)cinnamamide (compound 3), and N-(3-selenocyanatopropyl)cinnamamide (compound 4), were synthesized following two different approaches: direct conjugation and non-covalent immobilization onto hydrophilic and non-cytotoxic AuNPs functionalized with 3-mercapto-1-propanesulfonate (3MPS). Both free compounds and AuNPs-based systems were characterized via UV-Vis, FTIR NMR, mass spectrometry, and SR-XPS to assess their optical and structural properties. Size and colloidal stability were evaluated by DLS and ζ-potential measurements, whereas morphology at solid-state was evaluated by atomic force (AFM) and scanning electron (FESEM) microscopies. AuNPs synthesized through chemical reduction method in presence of Se-based compounds as functionalizing agents allowed the formation of aggregated NPs with little to no solubility in aqueous media. To improve their hydrophilicity and stability mixed AuNPs-3MPS-1 were synthesized. Besides, Se-loaded AuNPs-3MPS revealed to be the most suitable systems for biological studies in terms of size and colloidal stability. Selenium derivatives and AuNPs were tested in vitro via MTT assay against PC-3 (prostatic adenocarcinoma) and HCT-116 (colorectal carcinoma) cell lines. Compared to free compounds, direct functionalization onto AuNPs with formation of Au-Se covalent bond led to non-cytotoxic systems in the concentration range explored (0-100 μg/mL), whereas immobilization on AuNPs-3MPS improved the cytotoxicity of compounds 1, 3, and 4. Selective anticancer response against HCT-116 cells was obtained by AuNPs-3MPS-1. These results demonstrated that AuNPs can be used as a platform to tune the in vitro biological activity of organoselenium compounds.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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