Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.

Autor:	Bitzenhofer NL; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany., Hilgers F; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany., Bosio GN; Institute of Biological Information Processing IBI-1: Molecular and Cellular Physiology, Forschungszentrum Jülich GmbH, Jülich, Germany., Torra J; Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain., Casini G; Institute of Biological Information Processing IBI-1: Molecular and Cellular Physiology, Forschungszentrum Jülich GmbH, Jülich, Germany., Beinlich FRM; Institute of Biological Information Processing IBI-1: Molecular and Cellular Physiology, Forschungszentrum Jülich GmbH, Jülich, Germany., Knieps-Grünhagen E; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany., Gordeliy V; Institute of Bio-and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany.; Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-CEA-CNRS, Grenoble, France., Jaeger KE; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany.; Institute of Biological Information Processing IBI-1: Molecular and Cellular Physiology, Forschungszentrum Jülich GmbH, Jülich, Germany., Nonell S; Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain. santi.nonell@iqs.url.edu., Krauss U; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany. u.krauss@fz-juelich.de.; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany. u.krauss@fz-juelich.de., Gensch T; Institute of Biological Information Processing IBI-1: Molecular and Cellular Physiology, Forschungszentrum Jülich GmbH, Jülich, Germany. t.gensch@fz-juelich.de., Drepper T; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Jülich, Germany. t.drepper@fz-juelich.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2564, pp. 143-183.
DOI:	10.1007/978-1-0716-2667-2_7
Abstrakt:	Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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