Bone Sialoprotein Immobilized in Collagen Type I Enhances Bone Regeneration In vitro and In vivo.

Autor:	Kriegel A; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Schlosser C; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Habeck T; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Dahmen C; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Götz H; Cell Biology Unit, PKZI, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Clauder F; Immundiagnostik AG, Bensheim, Germany., Armbruster FP; Immundiagnostik AG, Bensheim, Germany., Baranowski A; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Drees P; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Rommens PM; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany., Ritz U; Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany.
Jazyk:	angličtina
Zdroj:	International journal of bioprinting [Int J Bioprint] 2022 Jul 12; Vol. 8 (3), pp. 591. Date of Electronic Publication: 2022 Jul 12 (Print Publication: 2022).
DOI:	10.18063/ijb.v8i3.591
Abstrakt:	The use of bioactive molecules is a promising approach to enhance the bone healing properties of biomaterials. The aim of this study was to define the role of bone sialoprotein (BSP) immobilized in collagen type I in various settings. In vitro studies with human primary osteoblasts in mono- or in co-culture with endothelial cells demonstrated a slightly increased gene expression of osteogenic markers as well as an increased proliferation rate in osteoblasts after application of BSP immobilized in collagen type I. Two critical size bone defect models were used to analyze bone regeneration. BSP incorporated in collagen type I increased bone regeneration only marginally at one concentration in a calvarial defect model. To induce the mechanical stability, three-dimensional printing was used to produce a stable porous cylinder of polylactide. The cylinder was filled with collagen type I and immobilized BSP and implanted into a femoral defect of critical size in rats. This hybrid material was able to significantly induce bone regeneration. Our study clearly shows the osteogenic effect of BSP when combined with collagen type I as carrier and thereby offers various approaches and options for its use as bioactive molecule in bone substitute materials. Competing Interests: Franz Paul Armbruster is the CEO of Immundiagnostik AG and Franziska Clauder is the employee of Immundiagnostik AG. All other authors state no conflicts of interest. (Copyright: © 2022 Kriegel, et al.)
Databáze:	MEDLINE
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