Electrohydrodynamic Jet-Printed Ultrathin Polycaprolactone Scaffolds Mimicking Bruch's Membrane for Retinal Pigment Epithelial Tissue Engineering.

Autor:	Liu H; Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore.; National University of Singapore (Suzhou) Research Institute, Suzhou, China., Wu F; National University of Singapore (Suzhou) Research Institute, Suzhou, China., Chen R; Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore.; National University of Singapore (Suzhou) Research Institute, Suzhou, China., Chen Y; Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore.; National University of Singapore (Suzhou) Research Institute, Suzhou, China., Yao K; Department of Mechatronic and Robotics, School of Advanced Technology, Xi'an JiaoTong-Liverpool University, Suzhou, China., Liu Z; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Parikh BH; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Jing L; Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore.; National University of Singapore (Suzhou) Research Institute, Suzhou, China., Liu T; National University of Singapore (Suzhou) Research Institute, Suzhou, China., Su X; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Sun J; Department of Mechatronic and Robotics, School of Advanced Technology, Xi'an JiaoTong-Liverpool University, Suzhou, China., Huang D; Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore.; National University of Singapore (Suzhou) Research Institute, Suzhou, China.
Jazyk:	angličtina
Zdroj:	International journal of bioprinting [Int J Bioprint] 2022 Apr 21; Vol. 8 (3), pp. 550. Date of Electronic Publication: 2022 Apr 21 (Print Publication: 2022).
DOI:	10.18063/ijb.v8i3.550
Abstrakt:	Age-related macular degeneration (AMD) is the leading cause of visual loss and affects millions of people worldwide. Dysfunction of the retinal pigment epithelium (RPE) is associated with the pathogenesis of AMD. The purpose of this work is to build and evaluate the performance of ultrathin scaffolds with an electrohydrodynamic jet (EHDJ) printing method for RPE cell culture. We printed two types of ultrathin (around 7 μm) polycaprolactone scaffolds with 20 μm and 50 μm pores, which possess mechanical properties resembling that of native human Bruch's membrane and are biodegradable. Light microscopy and cell proliferation assay showed that adult human retinal pigment epithelial (ARPE-19) cells adhered and proliferated to form a monolayer on the scaffolds. The progress of culture matured on the scaffolds was demonstrated by immunofluorescence (actin, ZO-1, and Na + /K + -ATPase) and Western blot analysis of the respective proteins. The RPE cells cultured on EHDJ-printed scaffolds with 20 μm pores presented higher permeability, higher transepithelial potential difference, and higher expression level of Na + /K + -ATPase than those cultured on Transwell inserts. These findings suggest that the EHDJ printing can fabricate scaffolds that mimic Bruch's membrane by promoting maturation of RPE cells to form a polarized and functional monolayered epithelium with potential as an in vitro model for studying retinal diseases and treatment methods. Competing Interests: The authors have no conflicts of interest to declare. (Copyright: © 2022 Liu, et al.)
Databáze:	MEDLINE
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